P4HA2 Antibodies
We offer P4HA2 Antibodies for use in common research applications: Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each P4HA2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our P4HA2 Antibodies can be used in a variety of model species: Human, Rat. Use the list below to choose the P4HA2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our P4HA2 polyclonal antibodies.
Alternate Names for P4HA2 Antibodies
anti-P4HA2 antibody, anti-2-oxoglutarate-4-dioxygenase subunit alpha-2 antibody, anti-4-PH alpha 2 antibody, anti-4-PH alpha-2 antibody, anti-alpha polypeptide II antibody, anti-C-P4Halpha(II) antibody, anti-collagen prolyl 4-hydroxylase alpha(II) antibody, anti-EC 1.14.11.2 antibody, anti-Procollagen-proline antibody, anti-procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase) antibody, anti-prolyl 4-hydroxylase subunit alpha-2 antibody, anti-prolyl 4-hydroxylase, alpha polypeptide II antibody
13 Results for "p4ha2" in Primary Antibodies
![Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Analysis in human epididymis and skeletal muscle tissues. Corresponding P4HA2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/P4HA2-Antibody-Immunohistochemistry-Paraffin-NBP2-33878-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Analysis in human epididymis and skeletal muscle tissues. Corresponding P4HA2 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: P4HA2 Antibody [NBP2-33878] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-P4HA2 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/P4HA2-Antibody-Western-Blot-NBP2-33878-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: P4HA2 Antibody [NBP2-33878] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/P4HA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33878-img0008.jpg)
![Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/P4HA2-Antibody-Immunohistochemistry-Paraffin-NBP2-33878-img0019.jpg)
![Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Staining of human epididymis shows high expression.](http://images.novusbio.com/fullsize/P4HA2-Antibody-Immunohistochemistry-Paraffin-NBP2-33878-img0010.jpg)
![Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/P4HA2-Antibody-Immunohistochemistry-Paraffin-NBP2-33878-img0016.jpg)
![Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/P4HA2-Antibody-Immunohistochemistry-Paraffin-NBP2-33878-img0017.jpg)
![Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33878] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/P4HA2-Antibody-Immunohistochemistry-Paraffin-NBP2-33878-img0018.jpg)
![Immunohistochemistry-Paraffin: P4HA2 Antibody (CL0351) [NBP2-52922] - Staining in human epididymis and skeletal muscle tissues. Corresponding P4HA2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/P4HA2-Antibody-CL0351-Immunohistochemistry-Paraffin-NBP2-52922-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: P4HA2 Antibody (CL0351) [NBP2-52922] - Staining in human epididymis and skeletal muscle tissues. Corresponding P4HA2 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: P4HA2 Antibody (CL0351) [NBP2-52922] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-P4HA2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/P4HA2-Antibody-CL0351-Western-Blot-NBP2-52922-img0008.jpg)
![Western Blot: P4HA2 Antibody (CL0351) [NBP2-52922] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/P4HA2-Antibody-CL0351-Western-Blot-NBP2-52922-img0006.jpg)
![Immunohistochemistry-Paraffin: P4HA2 Antibody (CL0351) [NBP2-52922] - Staining of human epididymis shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/P4HA2-Antibody-CL0351-Immunohistochemistry-Paraffin-NBP2-52922-img0010.jpg)
![Immunohistochemistry-Paraffin: P4HA2 Antibody (CL0351) [NBP2-52922] - Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/P4HA2-Antibody-CL0351-Immunohistochemistry-Paraffin-NBP2-52922-img0011.jpg)
![Immunohistochemistry-Paraffin: P4HA2 Antibody (CL0351) [NBP2-52922] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/P4HA2-Antibody-CL0351-Immunohistochemistry-Paraffin-NBP2-52922-img0012.jpg)
![Immunohistochemistry-Paraffin: P4HA2 Antibody (CL0351) [NBP2-52922] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/P4HA2-Antibody-CL0351-Immunohistochemistry-Paraffin-NBP2-52922-img0013.jpg)
![Immunoprecipitation: P4HA2 Antibody [NB110-40494] - Detection of Human P4HA2 on HeLa whole cell lysate using NB110-40494. P4HA2 was also immunoprecipitated by rabbit anti-P4HA2 antibodies NB100-40475 and another P4HA2 antibody.](http://images.novusbio.com/fullsize/P4HA2-Antibody-Immunoprecipitation-NB110-40494-img0002.jpg "Immunoprecipitation: P4HA2 Antibody [NB110-40494] - Detection of Human P4HA2 on HeLa whole cell lysate using NB110-40494. P4HA2 was also immunoprecipitated by rabbit anti-P4HA2 antibodies NB100-40475 and another P4HA2 antibody.")
![Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33909] - Staining in human epididymis and skeletal muscle tissues using anti-P4HA2 antibody. Corresponding P4HA2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/P4HA2-Antibody-Immunohistochemistry-Paraffin-NBP2-33909-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33909] - Staining in human epididymis and skeletal muscle tissues using anti-P4HA2 antibody. Corresponding P4HA2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: P4HA2 Antibody [NBP2-33909] - Analysis using Anti-P4HA2 antibody NBP2-33909 (A) shows similar pattern to independent antibody NBP2-33878 (B).](http://images.novusbio.com/fullsize/P4HA2-Antibody-Western-Blot-NBP2-33909-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: P4HA2 Antibody [NBP2-33909] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles.](http://images.novusbio.com/fullsize/P4HA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33909-img0003.jpg)
![Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33909] - Staining of human epididymis shows high expression.](http://images.novusbio.com/fullsize/P4HA2-Antibody-Immunohistochemistry-Paraffin-NBP2-33909-img0004.jpg)
![Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP2-33909] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/P4HA2-Antibody-Immunohistochemistry-Paraffin-NBP2-33909-img0005.jpg)
![Western Blot: P4HA2 Antibody [NBP3-06468] - Lane A: HeLa Whole Cell LysateLane B: H1299 Whole Cell LysateLane C: 293T Whole Cell LysateLane D: PC12 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:61 kDaObserved band size:61 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/P4HA2-Antibody-Western-Blot-NBP3-06468-img0001.jpg "Western Blot: P4HA2 Antibody [NBP3-06468] - Lane A: HeLa Whole Cell LysateLane B: H1299 Whole Cell LysateLane C: 293T Whole Cell LysateLane D: PC12 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:61 kDaObserved band size:61 kDa(We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: P4HA2 Antibody [NBP3-06468] - Staining of P4HA2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human P4HA2 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/P4HA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-06468-img0003.jpg)
![Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP3-06468] - Staining of human P4HA2 in human epididymis with rabbit polyclonal antibody at 1:2000 dilution.](http://images.novusbio.com/fullsize/P4HA2-Antibody-Immunohistochemistry-Paraffin-NBP3-06468-img0002.jpg)
![Immunoprecipitation: P4HA2 Antibody [NBP3-06468] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-P4HA2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-P4HA2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 61 kDaObserved band size :61 kDa](http://images.novusbio.com/fullsize/P4HA2-Antibody-Immunoprecipitation-NBP3-06468-img0004.jpg)
![Western Blot: P4HA2 Antibody [NBP3-38083] - Western blot analysis of lysates from Rat placenta, using P4HA2 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38083_rabbit-p4ha2-pab-10122024120642.jpg "Western Blot: P4HA2 Antibody [NBP3-38083] - Western blot analysis of lysates from Rat placenta, using P4HA2 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: P4HA2 Antibody [NBP3-38083] - Immunofluorescence analysis of NIH/3T3 cells using P4HA2 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38083_rabbit-p4ha2-pab-1012202411531081.jpg)
![Western Blot: P4HA2 Antibody [NBP3-38083] - Western blot analysis of various lysates using P4HA2 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38083_rabbit-p4ha2-pab-1012202411552860.jpg)
![Western Blot: P4HA2 Antibody (6A6Z2) [NBP3-33334] - Western blot analysis of various lysates, using P4HA2 Rabbit mAb at1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33334_rabbit-p4ha2-mab-6a6z2-10122024120673.jpg "Western Blot: P4HA2 Antibody (6A6Z2) [NBP3-33334] - Western blot analysis of various lysates, using P4HA2 Rabbit mAb at1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.")
![Western Blot: P4HA2 Antibody (6A6Z2) [NBP3-33334] - Western blot analysis of various lysates, using P4HA2 Rabbit mAb at1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33334_rabbit-p4ha2-mab-6a6z2-101220241233861.jpg)
![Western Blot: P4HA2 Antibody (C3) [NBP3-41655] - Human Placenta lysate; Primary Ab: 2ug/ml NBP3-41655; Second Ab: 0.2ug/mL HRP-conjugated Rabbit Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41655_mouse-p4ha2-mab-c3-14120251512376.jpg "Western Blot: P4HA2 Antibody (C3) [NBP3-41655] - Human Placenta lysate; Primary Ab: 2ug/ml NBP3-41655; Second Ab: 0.2ug/mL HRP-conjugated Rabbit Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: P4HA2 Antibody [NBP2-19665] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/P4HA2-Antibody-Western-Blot-NBP2-19665-img0001.jpg "Western Blot: P4HA2 Antibody [NBP2-19665] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: P4HA2 Antibody [NBP2-99486] - Anti-P4HA2 rabbit polyclonal antibody at 1:2000 dilution. Lane A: U251 MG Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: H1299 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa](http://images.novusbio.com/fullsize/P4HA2-Antibody-Western-Blot-NBP2-99486-img0002.jpg "Western Blot: P4HA2 Antibody [NBP2-99486] - Anti-P4HA2 rabbit polyclonal antibody at 1:2000 dilution. Lane A: U251 MG Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: H1299 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa")