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P4HA2 Antibody (CL0351) [Janelia Fluor® 525]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, KD
Clone
CL0351
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 525

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-52922AF350
Alexa Fluor 405
NBP2-52922AF405
Alexa Fluor 488
NBP2-52922AF488
Alexa Fluor 532
NBP2-52922AF532
Alexa Fluor 594
NBP2-52922AF594
Alexa Fluor 647
NBP2-52922AF647
Alexa Fluor 700
NBP2-52922AF700
Alexa Fluor 750
NBP2-52922AF750
Allophycocyanin
NBP2-52922APC
Biotin
NBP2-52922B
CoraFluor 1
NBP2-52922CL1
DyLight 350
NBP2-52922UV
DyLight 405
NBP2-52922V
DyLight 488
NBP2-52922G
DyLight 550
NBP2-52922R
DyLight 594
NBP2-52922DL594
DyLight 650
NBP2-52922C
DyLight 680
NBP2-52922FR
DyLight 755
NBP2-52922IR
FITC
NBP2-52922F
HRP
NBP2-52922H
Janelia Fluor 549
NBP2-52922JF549
Janelia Fluor 585
NBP2-52922JF585
Janelia Fluor 635
NBP2-52922JF635
Janelia Fluor 646
NBP2-52922JF646
Janelia Fluor 669
NBP2-52922JF669
PE
NBP2-52922PE
PerCP
NBP2-52922PCP
Unconjugated
NBP2-52922
mFluor Violet 450 SE
NBP2-52922MFV450
mFluor Violet 500 SE
NBP2-52922MFV500
mFluor Violet 610 SE
NBP2-52922MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-52922
Azide and BSA Free
NBP3-43972
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P4HA2 Antibody (CL0351) [Janelia Fluor® 525] Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LKEYILVEEAKLSKIKSWANKMEALTSKSAADAEGYLAHPVNAYKLVKRLNTDWPALEDLVLQDSAAGFIANLSVQRQFFPTDEDEIGAAKALMRLQDTYRLDPGTISRGELPGTKYQAMLSVDDCFGM
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
P4HA2
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for P4HA2 Antibody (CL0351) [Janelia Fluor® 525]

  • 2-oxoglutarate-4-dioxygenase subunit alpha-2
  • 4-PH alpha 2
  • 4-PH alpha-2
  • alpha polypeptide II
  • collagen prolyl 4-hydroxylase alpha(II)
  • C-P4Halpha(II)
  • EC 1.14.11.2
  • Procollagen-proline
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase)
  • prolyl 4-hydroxylase subunit alpha-2
  • prolyl 4-hydroxylase, alpha polypeptide II

Background

P4HA2 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol P4HA2