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P4HA1 Antibody - BSA Free

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Western Blot: P4HA1 Antibody [NBP1-84397] - Analysis using Anti-P4HA1 antibody NBP1-84397 (A) shows similar pattern to independent antibody NBP1-84398 (B).
Independent Antibodies: Western Blot: P4HA1 Antibody [NBP1-84397] - Analysis using Anti-P4HA1 antibody NBP1-84397 (A) shows similar pattern to independent antibody NBP1-84398 (B).
Immunocytochemistry/ Immunofluorescence: P4HA1 Antibody [NBP1-84397] - Staining of human cell line U-251 MG shows localization to endoplasmic reticulum.
Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397] - Staining of human skin shows weak granular cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397] - Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397] - Staining of human placenta shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397] - Staining in human placenta and pancreas tissues using anti-P4HA1 antibody. Corresponding P4HA1 RNA-seq data are presented for ...read more
Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397] - Staining of human endometrium shows moderate granular cytoplasmic positivity in in glandular cells.
Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397] - Staining of human kidney shows weak granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84397] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Validated by:
 

Independent Antibodies

      

Orthogonal Strategies

  

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-84397-25ul
Novus Biologicals offers 100% Guarantee

P4HA1 Antibody - BSA Free Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FTSIGQMTDLIHTEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLVLKDMSDGFISNLTIQRQYFPNDEDQVGAAKALLRLQDTYNLDTDTISKGNLPGVKHKSFLTAEDC
Predicted Species
Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
P4HA1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
P4HA1 Protein (NBP1-84397PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for P4HA1 Antibody - BSA Free

  • 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase)
  • 2-oxoglutarate-4-dioxygenase subunit alpha-1
  • 4-PH Alpha-1
  • alpha polypeptide I
  • EC 1.14.11.2
  • P4HA
  • P4HA1
  • Procollagen-proline
  • prolyl 4-hydroxylase, alpha polypeptide I

Background

P4HA1 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC

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Bioinformatics

Gene Symbol P4HA1