![Immunohistochemistry-Paraffin: p27/Kip1 Antibody (DCS-72.F6) [NBP2-44496] - Human Colon Carcinoma stained with p27 Monoclonal Antibody (DCS-72.F6)](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-DCS-72-F6-Immunohistochemistry-Paraffin-NBP2-44496-img0003.jpg "Immunohistochemistry-Paraffin: p27/Kip1 Antibody (DCS-72.F6) [NBP2-44496] - Human Colon Carcinoma stained with p27 Monoclonal Antibody (DCS-72.F6)")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, ICC/IF |
| Clone | DCS-72.F6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47770) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant full-length Mouse p27/Kip1 protein (Uniprot: P46527) |
| Epitope | aa 83-204 |
| Localization | Nuclear |
| Specificity | Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This monoclonal antibody co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CDKN1B |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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![Flow Cytometry: p27/Kip1 Antibody (DCS-72.F6) [NBP2-44496] - Human p27 on HeLa Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled p27 Monoclonal Antibody (DCS-72.F6).](http://images.novusbio.com/fullsize/p27-Kip1-Antibody-DCS-72-F6-Flow-Cytometry-NBP2-44496-img0001.jpg "Flow Cytometry: p27/Kip1 Antibody (DCS-72.F6) [NBP2-44496] - Human p27 on HeLa Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled p27 Monoclonal Antibody (DCS-72.F6).")
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