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p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) [FITC]

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Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF
Clone
DCS-72.F6 + KIP1/769
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47771AF350
Alexa Fluor 405
NBP2-47771AF405
Alexa Fluor 488
NBP2-47771AF488
Alexa Fluor 532
NBP2-47771AF532
Alexa Fluor 594
NBP2-47771AF594
Alexa Fluor 647
NBP2-47771AF647
Alexa Fluor 700
NBP2-47771AF700
Alexa Fluor 750
NBP2-47771AF750
Allophycocyanin
NBP2-47771APC
Biotin
NBP2-47771B
DyLight 350
NBP2-47771UV
DyLight 405
NBP2-47771V
DyLight 488
NBP2-47771G
DyLight 550
NBP2-47771R
DyLight 594
NBP2-47771DL594
DyLight 650
NBP2-47771C
DyLight 680
NBP2-47771FR
DyLight 755
NBP2-47771IR
HRP
NBP2-47771H
Janelia Fluor 549
NBP2-47771JF549
Janelia Fluor 646
NBP2-47771JF646
PE
NBP2-47771PE
PerCP
NBP2-47771PCP
Unconjugated
NBP2-44497-0.1mg
mFluor Violet 450 SE
NBP2-47771MFV450
mFluor Violet 500 SE
NBP2-47771MFV500
mFluor Violet 610 SE
NBP2-47771MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44497-0.1mg
Azide and BSA Free
NBP2-47771-0.1mg
IHC-Prediluted
NBP2-48401
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p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) [FITC] Summary

Immunogen
Mouse recombinant p27/Kip1 protein (DCS-72.F6); Recombinant human CDKN1B protein (KIP1/769) (Uniprot: P46527)
Epitope
aa 83-204
Localization
Nuclear
Specificity
Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This monoclonal antibody co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CDKN1B
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for p27/Kip1 Antibody (DCS-72.F6 + KIP1/769) [FITC]

  • CDKN1B
  • CDKN4
  • cyclin-dependent kinase inhibitor 1B (p27, Kip1)
  • cyclin-dependent kinase inhibitor 1B
  • Cyclin-dependent kinase inhibitor p27
  • Kip1
  • KIP1P27KIP1
  • MEN1B
  • MEN4
  • p27
  • p27Kip1

Background

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CDKN1B