![Immunocytochemistry/Immunofluorescence: OVOL2 Antibody (PCRP-OVOL2-2A1) [NBP3-07182] - Immunofluorescent analysis of PFA-fixed HeLa cells. OVOL2 Mouse Monoclonal Antibody (PCRP-OVOL2-2A1) followed by goat anti- Mouse IgG-CF488 (green); phalloidin counterstain (red).](http://images.novusbio.com/fullsize/OVOL2-Antibody-PCRP-OVOL2-2A1-Immunocytochemistry-Immunofluorescence-NBP3-07182-img0003.jpg "Immunocytochemistry/Immunofluorescence: OVOL2 Antibody (PCRP-OVOL2-2A1) [NBP3-07182] - Immunofluorescent analysis of PFA-fixed HeLa cells. OVOL2 Mouse Monoclonal Antibody (PCRP-OVOL2-2A1) followed by goat anti- Mouse IgG-CF488 (green); phalloidin counterstain (red).")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, MA |
| Clone | PCRP-OVOL2-2A1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08926) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant full-length human OVOL2 protein (Uniprot: Q9BRP0) |
| Localization | Nucleus. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | OVOL2 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Optimal dilution for a specific application should be determined. |
| Theoretical MW | 69.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
![Western Blot beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Western_Blot_5100.jpg)
![Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Chromatin_Immunoprecipitation_11251.jpg)
![Simple Western beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Flow Cytometry: OVOL2 Antibody (PCRP-OVOL2-2A1) [NBP3-07182] - Flow cytometric analysis of PFA-fixed HeLa cells. OVOL2 Mouse Monoclonal Antibody (PCRP-OVOL2-2A1) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).](http://images.novusbio.com/fullsize/OVOL2-Antibody-PCRP-OVOL2-2A1-Flow-Cytometry-NBP3-07182-img0002.jpg "Flow Cytometry: OVOL2 Antibody (PCRP-OVOL2-2A1) [NBP3-07182] - Flow cytometric analysis of PFA-fixed HeLa cells. OVOL2 Mouse Monoclonal Antibody (PCRP-OVOL2-2A1) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).")
![Protein Array: OVOL2 Antibody (PCRP-OVOL2-2A1) [NBP3-07182] - Analysis of Protein Array containing more than 19,000 full-length human proteins using OVOL2 Mouse Monoclonal Antibody (PCRP-OVOL2-2A1).](http://images.novusbio.com/fullsize/OVOL2-Antibody-PCRP-OVOL2-2A1-Protein-Array-NBP3-07182-img0001.jpg "Protein Array: OVOL2 Antibody (PCRP-OVOL2-2A1) [NBP3-07182] - Analysis of Protein Array containing more than 19,000 full-length human proteins using OVOL2 Mouse Monoclonal Antibody (PCRP-OVOL2-2A1).")
![Western Blot ID1 Antibody [Unconjugated]](https://images.novusbio.com/images2/ID1_AF4377_Western_Blot_5453.jpg)
![Immunocytochemistry ID1 Antibody [Unconjugated]](https://images.novusbio.com/images2/ID1_AF4377_Immunocytochemistry_12849.jpg)
![Simple Western ID1 Antibody [Unconjugated]](https://images.novusbio.com/images2/af4377_human-mouse-id1-affinity-purified-polyclonal-ab-simple-western-482021152036.jpg)
![Bioactivity Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images2/Sonic_Hedgehog_464SH_2840.jpg)
![SDS-PAGE Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images2/Sonic_Hedgehog_464-SH_341.jpg)
![SEC-MALS Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images/464-sh_recombinant-mouse-sonic-hedgehog-shh-c25ii-n-terminus-sec-mals-642023224324.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")