![Western Blot: ORAI3 Antibody [NBP2-76956] - Analysis of Orai3 using anti-Orai3 antibody at 1/500 dilution.Lane 1 : Raji cell lysatesLane 2 : Raji cell lysates(anti-Orai3 antibody+ immunizing peptide)](http://images.novusbio.com/fullsize/ORAI3-Antibody-Western-Blot-NBP2-76956-img0001.jpg "Western Blot: ORAI3 Antibody [NBP2-76956] - Analysis of Orai3 using anti-Orai3 antibody at 1/500 dilution.Lane 1 : Raji cell lysatesLane 2 : Raji cell lysates(anti-Orai3 antibody+ immunizing peptide)")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptide within Human ORAI3 aa 11-60 / 295. (SwissProt: Q9BRQ5 Human; SwissProt: Q6P8G8 Mouse; SwissProt: Q6AXR8 Rat) |
| Localization | Membrane. Multi-pass membrane protein. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ORAI3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 31 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.2% BSA, 50% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for ORAI3 Antibody (NBP2-76956)Find related products by research area.
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| Gene Symbol | ORAI3 |
![Immunocytochemistry/Immunofluorescence: ORAI3 Antibody [NBP2-76956] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Orai3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ORAI3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76956-img0008.jpg "Immunocytochemistry/Immunofluorescence: ORAI3 Antibody [NBP2-76956] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Orai3 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956] - Analysis of human placenta tissue using anti-Orai3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ORAI3-Antibody-Immunohistochemistry-NBP2-76956-img0009.jpg "Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956] - Analysis of human placenta tissue using anti-Orai3 antibody. Counter stained with hematoxylin.")
![Flow Cytometry: ORAI3 Antibody [NBP2-76956] - Analysis of A549 cells with Orai3 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/ORAI3-Antibody-Flow-Cytometry-NBP2-76956-img0010.jpg "Flow Cytometry: ORAI3 Antibody [NBP2-76956] - Analysis of A549 cells with Orai3 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.")
![Immunocytochemistry/Immunofluorescence: ORAI3 Antibody [NBP2-76956] - ICC staining Orai3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ORAI3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76956-img0002.jpg "Immunocytochemistry/Immunofluorescence: ORAI3 Antibody [NBP2-76956] - ICC staining Orai3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: ORAI3 Antibody [NBP2-76956] - ICC staining Orai3 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ORAI3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76956-img0003.jpg "Immunocytochemistry/Immunofluorescence: ORAI3 Antibody [NBP2-76956] - ICC staining Orai3 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: ORAI3 Antibody [NBP2-76956] - ICC staining Orai3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ORAI3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76956-img0004.jpg "Immunocytochemistry/Immunofluorescence: ORAI3 Antibody [NBP2-76956] - ICC staining Orai3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956] - Analysis of rat brain tissue using anti-Orai3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ORAI3-Antibody-Immunohistochemistry-NBP2-76956-img0005.jpg "Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956] - Analysis of rat brain tissue using anti-Orai3 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956] - Analysis of human liver cancer tissue using anti-Orai3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ORAI3-Antibody-Immunohistochemistry-NBP2-76956-img0006.jpg "Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956] - Analysis of human liver cancer tissue using anti-Orai3 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956] - Analysis of mouse testis tissue using anti-Orai3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ORAI3-Antibody-Immunohistochemistry-NBP2-76956-img0007.jpg "Immunohistochemistry-Paraffin: ORAI3 Antibody [NBP2-76956] - Analysis of mouse testis tissue using anti-Orai3 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry EphA3 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphA3_AF640_Immunohistochemistry_19184.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")