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Orai1 Antibody (3F6H5) - BSA Free

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Western Blot: Orai1 Antibody (3F6H5) [NBP1-75522] - analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at (A) 1 and (B) 2 ug/mL.
Western Blot: Orai1 Antibody (3F6H5) [NBP1-75522] - analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry-Paraffin: Orai1 Antibody (3F6H5) [NBP1-75522] - Human spleen tissue with ORAI1 antibody at 5 ug/mL.
Immunohistochemistry-Paraffin: Orai1 Antibody (3F6H5) [NBP1-75522] - ORAI1 in human spleen tissue with ORAI1 antibody at 2.5 ug/mL
Immunohistochemistry: Orai1 Antibody (3F6H5) [NBP1-75522] - ORAI1 in human spleen tissue with ORAI1 antibody at 20 ug/ml.
Immunohistochemistry: Orai1 Antibody (3F6H5) [NBP1-75522] - Human spleen tissue with ORAI1 antibody at 20 ug/ml. Red: ORAI1 Antibody [3F6H5] (NBP1-75522). Blue: DAPI staining

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Func, ICC/IF, IHC
Clone
3F6H5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-75522AF350
Alexa Fluor 405
NBP1-75522AF405
Alexa Fluor 488
NBP1-75522AF488
Alexa Fluor 532
NBP1-75522AF532
Alexa Fluor 594
NBP1-75522AF594
Alexa Fluor 647
NBP1-75522AF647
Alexa Fluor 700
NBP1-75522AF700
Alexa Fluor 750
NBP1-75522AF750
Biotin
NBP1-75522B
DyLight 350
NBP1-75522UV
DyLight 405
NBP1-75522V
DyLight 488
NBP1-75522G
DyLight 550
NBP1-75522R
DyLight 594
NBP1-75522DL594
DyLight 650
NBP1-75522C
DyLight 680
NBP1-75522FR
DyLight 755
NBP1-75522IR
FITC
NBP1-75522F
HRP
NBP1-75522H
Janelia Fluor 549
NBP1-75522JF549
Janelia Fluor 646
NBP1-75522JF646
PerCP
NBP1-75522PCP
mFluor Violet 450 SE
NBP1-75522MFV450
mFluor Violet 500 SE
NBP1-75522MFV500
mFluor Violet 610 SE
NBP1-75522MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-75522-0.025mg
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Orai1 Antibody (3F6H5) - BSA Free Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.
Specificity
ORAI1 antibody is predicted to have no cross reactivity to other members in the ORAI family.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ORAI1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • In-situ Hybridization
  • Proximity Ligation Assay
  • Western Blot 1-2 ug/ml
Application Notes
Use in In-situ Hybridization reported in scientific literature (PMID: 27535226). Use in Proximity ligation assay reported in scientific literature (PMID: 30018568). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:32199855).
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-75522 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Orai1 Antibody (3F6H5) - BSA Free

  • CRACM1
  • CRACM1ORAT1
  • FLJ14466
  • ORAI calcium release-activated calcium modulator 1
  • Orai1
  • ORAT1
  • Protein orai-1
  • TMEM142A
  • TMEM142Acalcium release-activated calcium modulator 1
  • Transmembrane protein 142Acalcium release-activated calcium channel protein 1

Background

Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one fo rm of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Orai1 Antibody
(3F6H5) - BSA Free
NBP1-75522
Orai1 Antibody (3F6H5) - BSA Free
Species: Hu, Mu, Rt
Applications: WB, ELISA, Func, ICC/IF, IHC

Publications for Orai1 Antibody (NBP1-75522)(4)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: IHC-Fr, IHC-P, ISH, PLA, WB.


Filter By Application
IHC-Fr
(1)
IHC-P
(1)
ISH
(1)
PLA
(1)
WB
(1)
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Human
(1)
Mouse
(2)
Rat
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ORAI1