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Nox4 Antibody [Biotin]

Images

 
Western Blot: NOX4 Antibody [Biotin] [NB110-58851B] - Detection of NOX4 in human kidney lysates uisng NB110-58851 at 0.5 ug/ml. Band observed at 31 kDa may represent reported splice isoform.
Immunohistochemistry: NOX4 Antibody [Biotin] [NB110-58851B] - Detection of NOX4 in proximal convoluted tubules of human kidney.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

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Nox4 Antibody [Biotin] Summary

Immunogen
A synthetic peptide made to a C-terminal region (within residues 500-578) of the human NOX4 protein sequence. [Swiss-Prot# Q9NPH5].
Localization
Endoplasmic reticulum, endoplasmic reticulum membrane, multi-pass membrane protein, cell membrane, nucleus (probable).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOX4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Nox4 Antibody [Biotin]

  • EC 1.6.3
  • Kidney oxidase-1
  • Kidney superoxide-producing NADPH oxidase
  • KOX
  • KOX-1
  • KOX-1Renal NAD(P)H-oxidase
  • NADPH oxidase 4
  • Nox4
  • RENOX
  • RENOXEC 1.6.3.-

Background

Oxygen sensing is essential for homeostasis in all aerobic organisms. A phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes, with resultant antimicrobial activity, has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex. NOX4 has a role as a redox messenger in the activation of intracellular signaling pathways leading (or contributing) to mitochondriogenesis, cell survival, and differentiation in hematopoietic stem cells. Data suggest that NOX4 provides a novel link between the insulin receptor and the generation of cellular reactive oxygen species that enhances insulin signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nox4 Antibody (NB110-58851B) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Nox4 Products

Research Areas for Nox4 Antibody (NB110-58851B)

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Blogs on Nox4.

NOX4 Antibodies: Don't NOX them until you've tried them
NOX4 is an NADPH oxidase that generates superoxide within the cell. It is primarily found in vascular cells, fibroblasts, and osteoclasts, with abundant expression in the kidney. Unlike its family members NOX1 and NOX2, NOX4 is constitutively active, ...  Read full blog post.

NOX4 Antibodies in Diabetic Nephropathy Research
Diabetic nephropathy (DN) is one of the leading complications resulting from chronic diabetes. It manifests as progressive renal failure caused by mesangial cell hyperplasia and fibrosis, and is one of the leading causes of terminal kidney disease (1)...  Read full blog post.

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Bioinformatics

Gene Symbol NOX4