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NOD1 Antibody (626919) [Unconjugated]

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Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human NOD1 or human NOD2, HT-29 human colon adenocarcinoma cell line, and SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NOD1 Monoclonal Antibody (Catalog # MAB7090) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NOD1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human NOD1 or human NOD2, HT-29 human colon adenocarcinoma cell line, and SW480 human colorectal ...read more
NOD1 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human NOD1 Monoclonal Antibody (Catalog # MAB7090) at 15 µg/mL overnight at 4 °C. Tissue was stained using the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
626919
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7090-SP
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NOD1 Antibody (626919) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human NOD1
Lys830-Phe953
Accession # Q9Y239
Specificity
Detects human NOD1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human NOD2 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NOD1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
MAB7090 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NOD1 Antibody (626919) [Unconjugated]

  • CARD4
  • CARD4NLR family, CARD domain containing 1
  • caspase recruitment domain family, member 4
  • Caspase recruitment domain-containing protein 4
  • CLR7.1
  • NLRC1
  • NOD1
  • nucleotide-binding oligomerization domain containing 1
  • nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 1
  • nucleotide-binding oligomerization domain-containing protein 1

Background

NOD1, also called CARD4, is a 108 kDa intracellular pattern recognition receptor of the Nod-like receptor (NLR or Caterpiller) family. This innate immune receptor recognizes the muramyl peptide, diaminopimelic acid. The 953 amino acid (aa) NOD1 contains a caspase recognition domain (CARD), an NTPase domain and 10 leucine‑rich repeats (LRR). Splicing variants of 925 and 779 aa lack all or part of LRR 7-10 and do not respond to muramyl peptides. Polymorphisms of NOD1 are associated with inflammatory bowel disease. Within aa 830-953 (LRR 7-110), human NOD1 shares 82% and 84% aa identity with mouse and rat NOD1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NOD1 Antibody
(626919) [Unconjugate
MAB7090
NOD1 Antibody (626919) [Unconjugated]
Species: Hu
Applications: WB, IHC

Publications for NOD1 Antibody (MAB7090)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, Western Blot.


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Product General Protocols

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Bioinformatics

Gene Symbol NOD1
Uniprot