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![Western Blot: NOD1 Antibody [NB100-56152] - Evo-induced apoptosis of HCC cells occurred via the NOD1 pathway in vivo. (A,B) The mice treated with or without 10 mg/kg of Evo. Levels of proteins in the NOD1 pathway in tumor tissues were detected by the Western blot method. Image collected and cropped by Citeab from the following publication (Evodiamine Induces Apoptosis in SMMC-7721 and HepG2 Cells by Suppressing NOD1 Signal Pathway. Int J Mol Sci (2018)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NOD1-Antibody-Western-Blot-NB100-56152-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: NOD1 Antibody [NB100-56152] - Evo-induced apoptosis of HCC cells occurred via the NOD1 pathway in vivo. (A,B) The mice treated with or without 10 mg/kg of Evo. Levels of proteins in the NOD1 pathway in tumor tissues were detected by the Western blot method. Image collected and cropped by Citeab from the following publication (Evodiamine Induces Apoptosis in SMMC-7721 and HepG2 Cells by Suppressing NOD1 Signal Pathway. Int J Mol Sci (2018)) licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: NOD1 Antibody [NB100-56152] - Immunocytochemistry/Immunofluorescence: NOD1 Antibody [NB100-56152] - Representative images from the immunofluorescence method (x400). HepG2 and SMMC-7721 cells were incubated with or without Evo (0, 0.5, and 1 uM) for 24 h to detect levels of NOD1 and p-P65. Values are means and standard errors of three separate experiments (* p < 0.05 and ** p < 0.01 versus control). Image collected and cropped by Citeab from the following publication (Evodiamine Induces Apoptosis in SMMC-7721 and HepG2 Cells by Suppressing NOD1 Signal Pathway. Int J Mol Sci (2018)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NOD1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56152-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: NOD1 Antibody [NB100-56152] - Evo-induced apoptosis of HCC cells occurred via the NOD1 pathway in vivo. (A,B) The mice treated with or without 10 mg/kg of Evo. Levels of proteins in the NOD1 pathway in tumor tissues were detected by the Western blot method. Image collected and cropped by Citeab from the following publication (Evodiamine Induces Apoptosis in SMMC-7721 and HepG2 Cells by Suppressing NOD1 Signal Pathway. Int J Mol Sci (2018)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NOD1-Antibody-Western-Blot-NB100-56152-img0004.jpg)
![Immunohistochemistry-Paraffin: NOD1 Antibody [NB100-56152] - Section of human sebaceous gland (A) and colon (B) stained for NOD1 expression using NB100-56152 at 1:2000. Hematoxylin counterstain. 10X magnification.](http://images.novusbio.com/fullsize/NOD1-Antibody-Immunohistochemistry-Paraffin-NB100-56152-img0002.jpg)
![Immunohistochemistry-Paraffin: NOD1 Antibody [NB100-56152] - Sections of human colon (A, A1) and pancreas (B, B1) stained for NOD1 expression using a DAB chromogen and Hematoxylin counterstain. Magnification: A (20X), A1 (40X). B (10X), B1 (40X).](http://images.novusbio.com/fullsize/NOD1-Antibody-Immunohistochemistry-Paraffin-NB100-56152-img0001.jpg)
![Western Blot: NOD1 Antibody [NB100-56152] - Evo induces apoptotic cell death of HepG2 & SMMC-7721 cells via the NOD1-mediated apoptotic pathway in vitro. HepG2 & SMMC-7721 cells were treated with 10 µg/mL IE-DAP for 2 h before exposure to 1 µM Evo. Apoptosis (A), cycle arrest (B), apoptosis-related protein levels (C) & the proteins’ levels of NOD1 pathway (D) in treated & untreated cells were measured by 5-ethynyl-2′-deoxyuridine (EdU) (×40), cellular propidium iodide (PI) fluorescence & Western blot methods. The percentage of proliferating cells (EdU+) was quantitated using ImageJ software (National Institutes of Health, Bethesda, MD, USA). Values are means & standard errors of three separate experiments (* p < 0.05 & ** p < 0.01 versus control, #p < 0.05 & ##p < 0.01 versus Evo). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30384473), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56152_rabbit-polyclonal-nod1-antibody-31020241534319.jpg)
![Western Blot: NOD1 Antibody [NB100-56152] - Evo-induced apoptosis of HCC cells occurred via the NOD1 pathway in vivo. (A,B) The mice treated with or without 10 mg/kg of Evo. Levels of proteins in the NOD1 pathway in tumor tissues were detected by the Western blot method. (C) Representative images from the immunofluorescence method. Expression of NOD1 (×400) & p-P65 (×200) in tumor tissues was analyzed by the immunofluorescence method. Values are means & standard errors of five separate experiments (* p < 0.05 & ** p < 0.01 versus control). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30384473), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56152_rabbit-polyclonal-nod1-antibody-310202415175228.jpg)
![Western Blot: NOD1 Antibody [NB100-56152] - Evo decreases expression of NOD1 resulting in suppression of nuclear factor-kappa B (NF-kappa B) & mitogen-activated protein kinase (MAPK) activation in vitro. (A) Expression of NOD1 in normal hepatocyte HL-7702, HepG2, & SMMC-7721 cells were detected by qRT-PCR & Western blot assays. (B) HepG2 & SMMC-7721 cells were incubated with 1 µM Evo for 0, 3, 6, & 12 h. The Western blot assay was performed to detect levels of proteins in the NOD1 pathway. (C) Representative images from the immunofluorescence method (×400). HepG2 & SMMC-7721 cells were incubated with or without Evo (0, 0.5, & 1 µM) for 24 h to detect levels of NOD1 & p-P65. Values are means & standard errors of three separate experiments (* p < 0.05 & ** p < 0.01 versus control). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30384473), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56152_rabbit-polyclonal-nod1-antibody-310202415293347.jpg)
![Western Blot: NOD1 Antibody [NB100-56878] - analysis of NOD1 in (A) recombinant fusion protein containing amino acids 930-949 and (B) fusion partner without these amino acids, using NOD1 antibody at 1:2500.](http://images.novusbio.com/fullsize/NOD1-Antibody-Western-Blot-NB100-56878-img0003.jpg "Western Blot: NOD1 Antibody [NB100-56878] - analysis of NOD1 in (A) recombinant fusion protein containing amino acids 930-949 and (B) fusion partner without these amino acids, using NOD1 antibody at 1:2500.")
![Immunohistochemistry-Paraffin: NOD1 Antibody [NB100-56878] - analysis of human pancreas using NOD1 antibody at 1:100.](http://images.novusbio.com/fullsize/NOD1-Antibody-Immunohistochemistry-Paraffin-NB100-56878-img0002.jpg)
![Recombinant Human NOD1 Protein [H00010392-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-NOD1-Protein-H00010392-P01-img0001.jpg "Recombinant Human NOD1 Protein [H00010392-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: NOD1 Antibody [NBP1-31349] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/NOD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31349-img0003.jpg "Immunocytochemistry/Immunofluorescence: NOD1 Antibody [NBP1-31349] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.")
![Western Blot: NOD1 Antibody [NBP1-31349] - NOD1 antibody [C2C3], C-term detects NOD1 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NOD1 antibody [C2C3], C-term (NBP1-31349) diluted at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp1-31349_rabbit-polyclonal-nod1-antibody-91020242075940.jpg)
![Western Blot: NOD1 Antibody [NBP1-76286] - EL4 cell lysate with NOD1 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/NOD1-Antibody-Western-Blot-NBP1-76286-img0001.jpg "Western Blot: NOD1 Antibody [NBP1-76286] - EL4 cell lysate with NOD1 antibody at 1 ug/mL.")
![Immunocytochemistry/Immunofluorescence: NOD1 Antibody [NBP2-57631] - Staining of human cell line HaCaT shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NOD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57631-img0001.jpg "Immunocytochemistry/Immunofluorescence: NOD1 Antibody [NBP2-57631] - Staining of human cell line HaCaT shows localization to mitochondria. Antibody staining is shown in green.")
![Western Blot: NOD1 Overexpression Lysate (Adult Normal) [NBL1-13705] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NOD1.](http://images.novusbio.com/fullsize/NOD1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13705-img0002.jpg "Western Blot: NOD1 Overexpression Lysate (Adult Normal) [NBL1-13705] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NOD1.")