Question about the Neurokinin 1 Receptor Antibody (NB300-101):why were the NK-1R in WB reported with different molecular mass(46kd, 50kd or about 70kd?)in publication 1,2 and 3? which is right?

Name: NK1R Antibody
Brand: Novus Biologicals
SKU: NB300-101
Rating: 4 (2 reviews)

Question

Accepted Answer

The theoretical MW of this protein is about 46KDa. Please see this reference for P25103 (https://www.uniprot.org/uniprotkb/P25103/entry). This is a protein database. The above MW weight is only theoretical and it can be expected that the MW differs between each experiment. The researcher who experienced this protein to be at 70KDa may not have reduced their protein enough which could have led to a protein complex, resulting in a larger MW.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, Gp, CaSpecies Glossary
Applications	WB, ICC/IF, IHC
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated

Immunogen	A synthetic peptide corresponding to the C-terminus of rat Neurokinin 1 Receptor, conjugated to bovine thyroglobulin. [UniProt# P14600]
Localization	In immunohistochemical experiments, plasma membrane staining is observed.
Specificity	NB300-101 is specific to the NK-1 receptor. Specificity was verified by the localization of immunohistochemical staining with NB300-101 in the neurons of mouse brain tissue.
Predicted Species	Canine (92%). Backed by our 100% Guarantee.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	TACR1
Purity	Unpurified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer	Whole antisera
Preservative	No Preservative
Purity	Unpurified

Publications using NB300-101	Applications	Species
Cui Y, Wang X, Xu Y et al. Ropivacaine promotes axon regeneration by regulating Nav1.8-mediated macrophage signaling after sciatic nerve injury in rats Anesthesiology 2023-09-05 [PMID: 37669448] (IHC-Fr, Rat)	IHC-Fr	Rat
Yanai R, Nishida T, Hatano M et al. Role of the Neurokinin-1 Receptor in the Promotion of Corneal Epithelial Wound Healing by the Peptides FGLM-NH2 and SSSR in Neurotrophic Keratopathy Investigative Opthalmology & Visual Science 2020-07-23 [PMID: 32697304] (Immunocytochemistry/ Immunofluorescence)	Immunocytochemistry/ Immunofluorescence
Lonndahl L, Rasul A, Lonne-Rahm SB et al. Tachykinin upregulation in atopic dermatitis Immunopharmacol Immunotoxicol 2019-02-18 [PMID: 30773959] (IF/IHC, Human)	IF/IHC	Human
Li Z, Luo T, Ning X et al. Neurokinin-1 receptor antagonism improves postoperative neurocognitive disorder in mice. Neuroscience Letters 2018-09-01 [PMID: 30273701] (WB, Mouse)	WB	Mouse
Li X, Guo R, Sun Y et al. Botulinum toxin type A and gabapentin attenuate postoperative pain and NK1 receptor internalization in rats. Neurochem. Int. 2018-03-20 [PMID: 29572051] (ICC/IF, Rat)	ICC/IF	Rat
JE Lim, E Chung, Y Son A neuropeptide, Substance-P, directly induces tissue-repairing M2 like macrophages by activating the PI3K/Akt/mTOR pathway even in the presence of IFN? Sci Rep, 2017-08-25;7(1):9417. 2017-08-25 [PMID: 28842601]
Morinaga R, Nakamuta N, Yamamoto Y. Hypoxia-induced increases in serotonin-immunoreactive nerve fibers in the medulla oblongata of the rat. Acta Histochem. 2016-10-01 [PMID: 27825705] (WB, Rat)	WB	Rat
Li W, Fotinos A, Wu Q et al. N-acetyl-l-tryptophan delays disease onset and extends survival in an amyotrophic lateral sclerosis transgenic mouse model. Neurobiol. Dis. 2015-05-16 [PMID: 25986728] (WB, Mouse)	WB	Mouse
Wu XN, Zhang T, Qian NS et al. Antinociceptive effects of endomorphin-2: Suppression of substance P release in the inflammatory pain model rat Neurochem. Int. 2015-02-07 [PMID: 25661513] (IHC-Fr, Rat) Details: NK1R antibody used for IHC-Fr application on Rat's spinal cord in experiments involving complete Freund's adjuvant/CFA inflammatory pain model - tissues removed after 7 days of CFA injection induced inflammation, paraformaldehyde perfusion, cryoprotection with 30% sucrose -0.1?M PBS pH?7.4, 10um cryosections, primary used at 1:1000 dilution, detection with FITC-labeled donkey anti-rabbit IgG secondary antibody (Fig.?6G and 6I )	IHC-Fr	Rat
Lin LH, Moore SA, Jones SY et al. Astrocytes in the rat nucleus tractus solitarii are critical for cardiovascular reflex control. J Neurosci. 2013-11-20 [PMID: 24259582] (ICC/IF, IHC-P, Rat)	ICC/IF, IHC-P	Rat
Yang Y, Yan M, Zhang H, Wang X. Substance P participates in immune-mediated hepatic injury induced by concanavalin A in mice and stimulates cytokine synthesis in Kupffer cells. Exp Ther Med. 2013-08-01 [PMID: 24137208] (ICC/IF, Mouse)	ICC/IF	Mouse
Luo YL, Li PB, Zhang CC et al. Effects of four antitussives on airway neurogenic inflammation in a guinea pig model of chronic cough induced by cigarette smoke exposure. Inflamm Res. 2013-12-01 [PMID: 24085318] (WB, Guinea Pig)	WB	Guinea Pig
Ramalho R, Almeida J, Fernandes R et al. Neurokinin-1 receptor, a new modulator of lymphangiogenesis in obese-asthma phenotype. Life Sci 2013-08-06 [PMID: 23792204] (IHC-P, Mouse)	IHC-P	Mouse
Talman WT, Dragon DN, Jones SY, Moore SA, Lin LH. Sudden Death and Myocardial Lesions after Damage to Catecholamine Neurons of the Nucleus Tractus Solitarii in Rat. Cell Mol Neurobiol. 2012-04-08 [PMID: 22484855] (IHC-Fr, ICC/IF, Rat)	IHC-Fr, ICC/IF	Rat
Grip L, Lonne-Rahm SB, Holst M, Johansson B, Nordlind K, Theodorsson E, El-Nour H. Substance P alterations in skin and brain of chronically stressed atopic-like mice. J Eur Acad Dermatol Venereol. 2012-01-18 [PMID: 22251186] (ICC/IF, IF/IHC, Mouse)	ICC/IF, IF/IHC	Mouse
Caioli S, Curcio L, Pieri M et al. Substance P receptor activation induces downregulation of the AMPA receptor functionality in cortical neurons from a genetic model of Amyotrophic Lateral Sclerosis. Neurobiol Dis. 2011-06-02 [PMID: 21726643] (ICC/IF, Mouse)	ICC/IF	Mouse
Commons KG. Locally collateralizing glutamate neurons in the dorsal raphe nucleus responsive to substance P contain vesicular glutamate transporter 3 (VGLUT3). J Chem Neuroanat 2009-12-01 [PMID: 19467322] (IF/IHC, ICC/IF, Rat)	IF/IHC, ICC/IF	Rat
Amadoro G, Pieri M, Ciotti MT et al. Substance P provides neuroprotection in cerebellar granule cells through Akt and MAPK/Erk activation: evidence for the involvement of the delayed rectifier potassium current. Neuropharmacology 2007-05-01 [PMID: 17397881] (Rat)		Rat
Broccardo M, Linari G, Agostini S et al. Expression of NK-1 and NK-3 tachykinin receptors in pancreatic acinar cells after acute experimental pancreatitis in rats. Am J Physiol Gastrointest Liver Physiol 2006-09-01 [PMID: 16782701] (ICC/IF, WB, Rat)	ICC/IF, WB	Rat
Broccardo M, Ciotti MT, Linari G et al. Immunocytochemical distribution of NK-1 and NK-3 tachykinin receptors in isolated pancreatic acini of guinea pigs and rats. Peptides 2005-11-01 [PMID: 15970359]
Commons KG, Connolley KR, Valentino RJ. A neurochemically distinct dorsal raphe-limbic circuit with a potential role in affective disorders. Neuropsychopharmacology 28(2):206-15. 2003-02-01 [PMID: 12589373] (ICC/IF, Rat)	ICC/IF	Rat
Kc P et al. Paraventricular vasopressin-containing neurons project to brain stem and spinal cord respiratory-related sites. Respir Physiol Neurobiol 133(1-2):75-88. 2002-10-23 [PMID: 12385733]
Mounir S, Parent A. The expression of neurokinin-1 receptor at striatal and pallidal levels in normal human brain. Neurosci Res 44(1):71-81. 2002-09-01 [PMID: 12204295] (IF/IHC, WB, Human)	IF/IHC, WB	Human
Ma MC et al. Temporal decrease in renal sensory responses in rats after chronic ligation of the bile duct. Am J Physiol Renal Physiol 283(1):F164-72. 2002-07-01 [PMID: 12060598] (WB, Rat)	WB	Rat
Yip, J et al. Localization of NK1 and NK3 receptors in guinea-pig brain. Regul Pept 98(1-2):55-62. 2001-04-02 [PMID: 11179779] (IF/IHC, Guinea Pig)	IF/IHC	Guinea Pig
Setou M, Nakagawa T, Seog DH, Hirokawa N. Kinesin superfamily motor protein KIF17 and mLin-10 in NMDA receptor-containing vesicle transport. Science 288(5472):1796-802. 2000-06-09 [PMID: 10846156]
Tooney, Paul A et al. Localisation of tachykinin NK1 and NK3 receptors in the human prefrontal and visual cortex. Neurosci Lett;283(3):185-8. 2000-04-14 [PMID: 10754218] (IF/IHC, Human)	IF/IHC	Human
Anderson LE, Seybold VS. Phosphorylated cAMP response element binding protein increases in neurokinin-1 receptor-immunoreactive neurons in rat spinal cord in response to formalin-induced nociception. Neurosci Lett 283(1):29-32. 2000-03-31 [PMID: 10729626] (IF/IHC, Rat)	IF/IHC	Rat
Davis BJ, Smith HM. Neurokinin-1 receptor immunoreactivity in the nucleus of the solitary tract in the hamster. Neuroreport 1999-04-06 [PMID: 10321475]
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Gene Symbol	TACR1
Entrez	6869(Human) 24807(Rat)
Uniprot	P14600(Rat)

	Li-Hsien Lin 12/20/2010
Application:	IF
Species:	Rat

	Li-Hsien Lin 09/09/2010
Application:	IF
Species:	Rat