![Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (7D3) [H00001138-M01] - Analysis of CHRNA5 expression in transfected 293T cell line by CHRNA5 monoclonal antibody (M01), clone 7D3.Lane 1: CHRNA5 transfected lysate (Predicted MW: 53.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-5-CHRNA5-Antibody-7D3-Western-Blot-H00001138-M01-img0002.jpg "Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (7D3) [H00001138-M01] - Analysis of CHRNA5 expression in transfected 293T cell line by CHRNA5 monoclonal antibody (M01), clone 7D3.Lane 1: CHRNA5 transfected lysate (Predicted MW: 53.1 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 7D3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | CHRNA5 (NP_000736, 38 a.a. ~ 131 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SEPSSIAKHEDSLLKDLFQDYERWVRPVEHLNDKIKIKFGLAISQLVDVDEKNQLMTTNVWLKQEWIDVKLRWNPDDYGGIKVIRVPSDSVWTP |
| Specificity | CHRNA5 - cholinergic receptor, nicotinic, alpha polypeptide 5 |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CHRNA5 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (H00001138-M01)Find related products by research area.
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![Sandwich ELISA: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (7D3) [H00001138-M01] - Detection limit for recombinant GST tagged CHRNA5 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-R-alpha-5-CHRNA5-Antibody-7D3-Sandwich-ELISA-H00001138-M01-img0001.jpg "Sandwich ELISA: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (7D3) [H00001138-M01] - Detection limit for recombinant GST tagged CHRNA5 is 0.3 ng/ml as a capture antibody.")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")