![Western Blot: NFkB1/NFkB p105 Antibody [NBP1-77395] - WB analysis of NFkB in HepG2 whole cell lysate.](http://images.novusbio.com/fullsize/NFkB1-NFkB-p105-Antibody-Western-Blot-NBP1-77395-img0003.jpg "Western Blot: NFkB1/NFkB p105 Antibody [NBP1-77395] - WB analysis of NFkB in HepG2 whole cell lysate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | A genomic peptide made to an N-terminal region of the human NFkB p105/p50 protein (within residues 400-590). [Swiss-Prot P19838] |
| Localization | Nucleus and cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NFKB1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Western blot, a band is seen approx. 110 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 105 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS and 30% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: NFkB1/NFkB p105 Antibody [NBP1-77395] - Antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/NFkB1-NFkB-p105-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77395-img0004.jpg "Immunocytochemistry/Immunofluorescence: NFkB1/NFkB p105 Antibody [NBP1-77395] - Antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).")
![Immunohistochemistry: NFkB1/NFkB p105 Antibody [NBP1-77395] - IHC analysis of NFKB in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/NFkB1-NFkB-p105-Antibody-Immunohistochemistry-NBP1-77395-img0005.jpg "Immunohistochemistry: NFkB1/NFkB p105 Antibody [NBP1-77395] - IHC analysis of NFKB in mouse kidney using DAB with hematoxylin counterstain.")
![Simple Western: NFkB1/NFkB p105 Antibody [NBP1-77395] - Simple Western lane view shows a specific band for NFkB p105 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NFkB1-NFkB-p105-Antibody-Simple-Western-NBP1-77395-img0006.jpg "Simple Western: NFkB1/NFkB p105 Antibody [NBP1-77395] - Simple Western lane view shows a specific band for NFkB p105 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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