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NFkB1/NFkB p105 Antibody [Unconjugated]

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Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse NF kappa B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2697) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for NF kappa B1 at approximately 50 and 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse NF kappa B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2697) ...read more
Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. NF kappa ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2697-SP
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NFkB1/NFkB p105 Antibody [Unconjugated] Summary

Specificity
Detects endogenous human and mouse NF kappa B1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NFKB1
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Western Blot 0.5 ug/mL
Publications
Read Publication using AF2697.

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NFkB1/NFkB p105 Antibody [Unconjugated]

  • DNA-binding factor KBF1
  • EBP-1
  • NF-kappa-B
  • NFkB1
  • NF-kB1
  • NFKB-p105
  • Nuclear factor NF-kappa-B p105 subunit
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
  • p105

Background

Nuclear Factor kappa B1 (NF kappa B1 or NF kappa B p50) is a member of the rel/NF kappa B family of transcription factors. NF kappa B1 dimerizes with other members of the rel/NF kappa B family to regulate expression of genes that participate in immune, apoptotic, and oncogenic processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NFkB1/NFkB p105 Antibody (AF2697)(1)


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Bioinformatics

Gene Symbol NFKB1
Uniprot