![Western Blot: NFIA Antibody (1E11) [H00004774-M02] - Analysis of NFIA expression in transfected 293T cell line by NFIA monoclonal antibody (M02), clone 1E11.Lane 1: NFIA transfected lysate (Predicted MW: 54.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NFIA-Antibody-1E11-Western-Blot-H00004774-M02-img0005.jpg "Western Blot: NFIA Antibody (1E11) [H00004774-M02] - Analysis of NFIA expression in transfected 293T cell line by NFIA monoclonal antibody (M02), clone 1E11.Lane 1: NFIA transfected lysate (Predicted MW: 54.6 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 1E11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | NFIA (AAH22264, 1 a.a. ~ 498 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MYSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKEEERAVKDELLSEKPEVKQKWASRLLAKLRKDIRPEYREDFVLTVTGKKPPCCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKSPQCSNPGLCVQPHHIGVSVKELDLYLAYFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFVTSGVFSVTELVRVSQTPIAAGTGPNFSLSDLESSSYYSMSPGAMRRSLPSTSSTSSTKRLKSVEDEMDSPGEEPFYTGQGRSPGSGSQSSGWHEVEPGMPSPTTLKKSEKSGFSSPSPSQTSSLGTAFTQHHRPVITGPRASPHATPSTLHFPTSPIIQQPGPYFSHPAIRYHPQETLKEFVQLVCPDAGQQAGQVGFLNPNGSSQGKVHNPFLPTPMLPPPPPPPMARPVPLPVPDTKPPTTSTEGGAASPTSPILVPGIKVAASHHPPDRPPDPFSTL |
| Specificity | NFIA - nuclear factor I/A (1E11) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NFIA |
| Purity | Ascites |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | Ascites |
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Secondary Antibodies |
Isotype Controls |
Research Areas for NFIA Antibody (H00004774-M02)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: NFIA Antibody (1E11) [H00004774-M02] - Analysis of monoclonal antibody to NFIA on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NFIA-Antibody-1E11-Immunocytochemistry-Immunofluorescence-H00004774-M02-img0003.jpg "Immunocytochemistry/Immunofluorescence: NFIA Antibody (1E11) [H00004774-M02] - Analysis of monoclonal antibody to NFIA on HeLa cell . Antibody concentration 10 ug/ml.")
![Sandwich ELISA: NFIA Antibody (1E11) [H00004774-M02] - Detection limit for recombinant GST tagged NFIA is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NFIA-Antibody-1E11-Sandwich-ELISA-H00004774-M02-img0004.jpg "Sandwich ELISA: NFIA Antibody (1E11) [H00004774-M02] - Detection limit for recombinant GST tagged NFIA is approximately 0.3ng/ml as a capture antibody.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")