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NFIX Antibody

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Immunocytochemistry/ Immunofluorescence: NFIX Antibody [NBP2-15039] - Nfix is mainly expressed by anti-inflammatory MPs. Percentage of F4/80+ MPs positive for Nfix in Tibialis Anterior muscles (TA) of WT mice injected ...read more
Immunohistochemistry-Paraffin: NFIX Antibody [NBP2-15039] - Mouse hippocampus.NFIX stained by NFIX antibody [N3C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: NFIX Antibody [NBP2-15039] - Mouse hippocampus.NFIX stained by NFIX antibody [N3C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Product Details

Summary
Reactivity Hu, Mu, XpSpecies Glossary
Applications WB, ICC/IF, IHC, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NFIX Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NFIX. The exact sequence is proprietary.
Predicted Species
Xenopus (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFIX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) Reported in scientific literature (PMID: 30266829).
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID: 30266829).
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot Reported in scientific literature (PMID: 30266829).
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-15039 in the following applications:

Reactivity Notes

Xenopus laevis (90%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for NFIX Antibody

  • CTF
  • NF1ACCAAT-box-binding transcription factor
  • NF1-X
  • NF-I/X
  • NFI-X
  • nuclear factor 1 X-type
  • Nuclear factor 1/X
  • nuclear factor I/X (CCAAT-binding transcription factor)
  • Nuclear factor I/X
  • TGGCA-binding protein

Background

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for NFIX Antibody (NBP2-15039)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Chemotaxis, ICC/IF, IF/IHC, WB.


Filter By Application
Chemotaxis
(2)
ICC/IF
(2)
IF/IHC
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
All Species
Showing Publications 1 - 9 of 9.
Publications using NBP2-15039 Applications Species
Ribeiro VFL How Lama2-deficiency impacts myoblast differentiation in a mouse model for LAMA2-congenital muscular dystrophy Thesis 2022-01-01 (ICC/IF, Mouse, Human) ICC/IF Mouse, Human
Martins SG, Ribeiro V, Melo C et al. Laminin-alpha 2 chain deficiency in skeletal muscle causes dysregulation of multiple cellular mechanisms Life Science Alliance 2024-10-08 [PMID: 39379105]
Ishikawa A, Fukui T, Kido A et al. Discovering cancer stem‐like molecule, nuclear factor I X, using spatial transcriptome in gastric cancer Cancer Science 2024-07-17 [PMID: 39021298]
Ge R, Wang C, Liu J et al. A Novel Tumor-Promoting Role for Nuclear Factor IX in Glioblastoma Is Mediated through Transcriptional Activation of GINS1 Molecular Cancer Research 2022-12-28 [PMID: 36469009]
Liu Z, Ge R, Zhou J et al. Nuclear factor IX promotes glioblastoma development through transcriptional activation of Ezrin Oncogenesis 2020-04-14 [PMID: 32291386] (Chemotaxis, Mouse) Chemotaxis Mouse
Saclier M, Lapi M, Bonfanti C et Al. The Transcription Factor Nfix Requires RhoA-ROCK1 Dependent Phagocytosis to Mediate Macrophage Skewing during Skeletal Muscle Regeneration Cells 2020-03-13 [PMID: 32183151]
Taglietti V, Angelini G, Mura G et al. RhoA and ERK signalling regulate the expression of the myogenic transcription factor Nfix. Development 2018-09-28 [PMID: 30266829] (Chemotaxis, ICC/IF, WB, Mouse) Chemotaxis, ICC/IF, WB Mouse
Zhou F, Yuan Q, Zhang W et al. MiR-663a Stimulates Proliferation and Suppresses Early Apoptosis of Human Spermatogonial Stem Cells by Targeting NFIX and Regulating Cell Cycle. Molecular Therapy - Nucleic Acids 2018-09-01 [PMID: 30195770]
Taglietti V, Maroli G, Cermenati S et al. Nfix Induces a Switch in Sox6 Transcriptional Activity to Regulate MyHC-I Expression in Fetal Muscle. Cell Rep. 2016-11-22 [PMID: 27880909] (IF/IHC, Mouse) IF/IHC Mouse

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NFIX
Uniprot