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Rat NF-L ELISA Kit (Colorimetric)

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ELISA: Rat NF-L ELISA Kit (Colorimetric) [NBP2-81185] - Samples were spiked with high concentrations of Rat NF-L and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Rat NF-L ELISA Kit (Colorimetric) [NBP2-81185] - Standard Curve Reference
ELISA: Rat NF-L ELISA Kit (Colorimetric) [NBP2-81185] - Image from a verified customer review.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL

Order Details

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Rat NF-L ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat NF-L in samples. No significant cross-reactivity or interference between Rat NF-L and analogues was observed.
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.4%
Intra-Assay
CV% < 5.36%
Spike Recovery
87-102%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
NEFL

Applications/Dilutions

Dilutions
  • ELISA
Reviewed Applications
Read 1 Review rated 5
using
NBP2-81185 in the following application:

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat NF-L ELISA Kit (Colorimetric)

  • 68 kDa neurofilament protein
  • CMT2E
  • NEFL
  • neurofilament protein, light chain
  • neurofilament subunit NF-L
  • Neurofilament triplet L protein
  • neurofilament, light polypeptide
  • neurofilament-light
  • NF68
  • NF68FLJ53642
  • NFL
  • NF-L
  • NFLlight polypeptide 68kDa

Background

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-L is the light or low molecular weight polypeptide, and it runs on SDS-PAGE gels at about 68kDa, with some size variation in across species.

Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for NF-L ELISA Kit (NBP2-81185) (0)

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Review for NF-L ELISA Kit (NBP2-81185) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBP2-81185:
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Verified Customer
04/17/2024
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Summary

Sample TestedBlood plasma
LotPA18448H6020

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FAQs for NF-L ELISA Kit (NBP2-81185) (0)

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Verified Customer
04/17/2024
Application:
Species:

Bioinformatics

Gene Symbol NEFL