Rat NF-L ELISA Kit (Colorimetric) Summary
Specificity |
This kit recognizes Rat NF-L in samples. No significant cross-reactivity or interference between Rat NF-L and analogues was observed. |
Standard Curve Range |
15.63 - 1000 pg/mL |
Sensitivity |
9.38 pg/mL |
Assay Type |
Sandwich-ELISA |
Inter-Assay |
CV% < 4.4% |
Intra-Assay |
CV% < 5.36% |
Spike Recovery |
87-102% |
Sample Volume |
100 ul |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
NEFL |
Applications/Dilutions
Dilutions |
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Reviewed Applications |
Read 1 Review rated 5 using NBP2-81185 in the following application:
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Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
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- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
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Alternate Names for Rat NF-L ELISA Kit (Colorimetric)
Background
Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-L is the light or low molecular weight polypeptide, and it runs on SDS-PAGE gels at about 68kDa, with some size variation in across species.
Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Publications for NF-L ELISA Kit (NBP2-81185) (0)
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Review for NF-L ELISA Kit (NBP2-81185) (1)
51
Average Rating: 5 (Based on 1 review)
Reviews using NBP2-81185:
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Ratings |
Applications |
Species |
Date |
Details |
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5
reviewed by: Verified Customer
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04/17/2024 |
Summary
Sample Tested | Blood plasma | Lot | PA18448H6020 |
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Product General Protocols
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FAQs for NF-L ELISA Kit (NBP2-81185) (0)
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