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Human NF-L ELISA Kit (Colorimetric)

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ELISA: Human NF-L ELISA Kit (Colorimetric) [NBP2-81184] - Samples were spiked with high concentrations of Human NF-L and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Human NF-L ELISA Kit (Colorimetric) [NBP2-81184] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL

Order Details

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Human NF-L ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human NF-L in samples. No significant cross-reactivity or interference between Human NF-L and analogues was observed.
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.92%
Intra-Assay
CV% < 4.94%
Spike Recovery
86-106%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
NEFL

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publications using
NBP2-81184 in the following applications:

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human NF-L ELISA Kit (Colorimetric)

  • 68 kDa neurofilament protein
  • CMT2E
  • NEFL
  • neurofilament protein, light chain
  • neurofilament subunit NF-L
  • Neurofilament triplet L protein
  • neurofilament, light polypeptide
  • neurofilament-light
  • NF68
  • NF68FLJ53642
  • NFL
  • NF-L
  • NFLlight polypeptide 68kDa

Background

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-L is the light or low molecular weight polypeptide, and it runs on SDS-PAGE gels at about 68kDa, with some size variation in across species.

Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for NF-L ELISA Kit (NBP2-81184)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for NF-L ELISA Kit (NBP2-81184). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in your elisa kit NBP2-81184 for NF-L test. Can I use cerebrospinal fluid as the semple of my test? is it gonna affect the accuracy of the test with your kit?
    • This kit is validated for biological fluids. However, you should validate your sample type, perhaps by performing spike and recovery.

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Bioinformatics

Gene Symbol NEFL