ELISA: Human NF-L ELISA Kit (Colorimetric) [NBP2-81184] - Samples were spiked with high concentrations of Human NF-L and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Human NF-L ELISA Kit (Colorimetric) [NBP2-81184] - Standard Curve Reference
Product Details
Summary
Reactivity
HuSpecies Glossary
Applications
ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Storage of components varies. See protocol for specific instructions.
Kit Components
Components
Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent
Alternate Names for Human NF-L ELISA Kit (Colorimetric)
68 kDa neurofilament protein
CMT2E
NEFL
neurofilament protein, light chain
neurofilament subunit NF-L
Neurofilament triplet L protein
neurofilament, light polypeptide
neurofilament-light
NF68
NF68FLJ53642
NFL
NF-L
NFLlight polypeptide 68kDa
Background
Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-L is the light or low molecular weight polypeptide, and it runs on SDS-PAGE gels at about 68kDa, with some size variation in across species.
Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
FAQs for NF-L ELISA Kit (NBP2-81184). (Showing 1 - 1 of 1 FAQs).
I am interested in your elisa kit NBP2-81184 for NF-L test. Can I use cerebrospinal fluid as the semple of my test? is it gonna affect the accuracy of the test with your kit?
This kit is validated for biological fluids. However, you should validate your sample type, perhaps by performing spike and recovery.