Mouse NF-L ELISA Kit (Colorimetric)

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ELISA: Mouse NF-L ELISA Kit (Colorimetric) [NBP2-80299] - Samples were spiked with high concentrations of Mouse NF-L and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Mouse NF-L ELISA Kit (Colorimetric) [NBP2-80299] - Standard Curve Reference
ELISA: Mouse NF-L ELISA Kit (Colorimetric) [NBP2-80299] - Image from a verified customer review.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.156-10 ng/mL
Sensitivity
0.094 ng/mL

Order Details

Mouse NF-L ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.156-10 ng/mL
Sensitivity
0.094 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.95%
Intra-Assay
CV% < 4.85%
Spike Recovery
88-105%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
NEFL

Applications/Dilutions

Dilutions
  • ELISA
Reviewed Applications
Read 1 Review rated 5
using
NBP2-80299 in the following application:

Publications
Read Publications using NBP2-80299.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25X)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse NF-L ELISA Kit (Colorimetric)

  • 68 kDa neurofilament protein
  • CMT2E
  • NEFL
  • neurofilament protein, light chain
  • neurofilament subunit NF-L
  • Neurofilament triplet L protein
  • neurofilament, light polypeptide
  • neurofilament-light
  • NF68
  • NF68FLJ53642
  • NFL
  • NF-L
  • NFLlight polypeptide 68kDa

Background

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-L is the light or low molecular weight polypeptide, and it runs on SDS-PAGE gels at about 68kDa, with some size variation in across species.

Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for NF-L ELISA Kit (NBP2-80299)(2)

We have publications tested in 1 application: ELISA.


Filter By Application
ELISA
(2)
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Showing Publications 1 - 2 of 2.
Publications using NBP2-80299 Applications Species
Berthiaume A, Reda S, Johnston J et al. ATH-1105, a Small Molecule Positive Modulator of HGF/MET, is Neuroprotective and Preserves Motor and Nerve Function in a TDP-43 Mouse Model of ALS Cell Reports Medicine 2023-02-22 (ELISA) ELISA
Jalnapurkar B, Le D, Shainin W et al. A deep-learning-based screening platform in aging-relevant human motor neurons to identify therapeutic compounds for amyotrophic lateral sclerosis bioRxiv 2023-11-22 (ELISA) ELISA

Review for NF-L ELISA Kit (NBP2-80299) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBP2-80299:
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
04/17/2024
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Summary

Sample TestedBlood plasma
LotZD22F4JJ6187

Product General Protocols

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FAQs for NF-L ELISA Kit (NBP2-80299) (0)

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Verified Customer
04/17/2024
Application:
Species:

Bioinformatics

Gene Symbol NEFL