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NF-L Antibody (CL4729)

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Western Blot: NF-L Antibody (CL4729) [NBP2-59061] - Analysis in human cerebral cortex tissue.
Immunocytochemistry/ Immunofluorescence: NF-L Antibody (CL4729) [NBP2-59061] - Staining of PC-3 cells using the Anti-NEFL monoclonal antibody, showing specific staining in intermediate filaments in green. Microtubule- ...read more
Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of human cerebral cortex shows strong positivity in neural fibers.
Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of rat cerebral cortex shows strong immunoreactivity in neuronal cell bodies and processes.
Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of mouse cerebral cortex shows strong immunoreactivity in neuronal cell bodies and processes.
Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061] - Staining of human cerebellum shows strong cytoplasmic immunoreactivity in cell bodies and proximal dendrites of Purkinje cells.
Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of rat brainstem shows strong immunoreactivity in neuronal cell bodies.
Immunohistochemistry: NF-L Antibody (CL4729) [NBP2-59061] - NF-L Antibody [NBP2-59061] - Staining of mouse striatum shows strong positivity in neural fibers.
Immunohistochemistry-Paraffin: NF-L Antibody (CL4729) [NBP2-59061] - Staining of human liver shows no positivity as expected (negative control).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL4729
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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NF-L Antibody (CL4729) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:SSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRF
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NEFL
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:10000 - 1:20000
  • Immunohistochemistry-Paraffin 1:10000 - 1:20000
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Control Peptide
NF-L Recombinant Protein Antigen (NBP2-59061PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for NF-L Antibody (CL4729)

  • 68 kDa neurofilament protein
  • CMT2E
  • NEFL
  • neurofilament protein, light chain
  • neurofilament subunit NF-L
  • Neurofilament triplet L protein
  • neurofilament, light polypeptide
  • neurofilament-light
  • NF68
  • NF68FLJ53642
  • NFL
  • NF-L
  • NFLlight polypeptide 68kDa

Background

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-L is the light or low molecular weight polypeptide, and it runs on SDS-PAGE gels at about 68kDa, with some size variation in across species.

Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NEFL