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NF-L Antibody (1002615) [Unconjugated]

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Western blot shows lysates of human motor cortex tissue, mouse hypothalamus tissue, and rat hypothalamus tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat NF-L Monoclonal Antibody (Catalog # ...read more
NF‑L was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human/Mouse/Rat NF‑L Monoclonal Antibody (Catalog # MAB22163) at 1.7 µg/mL for 1 hour at room ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
1002615
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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NF-L Antibody (1002615) [Unconjugated] Summary

Immunogen
68kD purified NF-L from Bovine spinal cord.
Specificity
Detects human NF-L in direct ELISAs. Detects human, mouse, and rat NF-L in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publication using
MAB22163 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NF-L Antibody (1002615) [Unconjugated]

  • 68 kDa neurofilament protein
  • CMT2E
  • NEFL
  • neurofilament protein, light chain
  • neurofilament subunit NF-L
  • Neurofilament triplet L protein
  • neurofilament, light polypeptide
  • neurofilament-light
  • NF68
  • NF68FLJ53642
  • NFL
  • NF-L
  • NFLlight polypeptide 68kDa

Background

NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that is expressed in neurons. It associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NF-L Antibody (MAB22163)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Simple Western.


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