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NeuroD1 Antibody (3H8)

Images

 
Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody (M01), clone 3H8.Lane 1: NEUROD1 transfected lysate(39.9 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of monoclonal antibody to NEUROD1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of monoclonal antibody to NEUROD1 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml.
Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01] - Western Blot analysis of NEUROD1 expression in IMR-32 ( Cat # L008V1 ).
ELISA: NeuroD1 Antibody (3H8) [H00004760-M01] - Detection limit for recombinant GST tagged NEUROD1 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
3H8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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NeuroD1 Antibody (3H8) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
NEUROD1 (AAH09046, 201 a.a. ~ 300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QDMPPHLPTASASFPVHPYSYQSPGLPSPPYGTMDSSHVFHVKPPPHAYSAALEPFFESPLTDCTSPSFDGPLSPPLSINGNFSFKHEPSAEFEKNYAFT
Specificity
NEUROD1 - neurogenic differentiation 1
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NEUROD1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
Application Notes
Antibody reactivity against recominant protein, transfected lysate and cell lysate for WB. Use in Immunohistochemistry reported in scientific literature (PMID: 27320912).
Publications
Read Publications using
H00004760-M01 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27320912).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NeuroD1 Antibody (3H8)

  • basic helix-loop-helix transcription factor
  • BETA2
  • BHF-1
  • BHLHA3
  • bHLHa3beta-cell E-box transactivator 2
  • Class A basic helix-loop-helix protein 3
  • MODY6
  • NeuroD
  • NeuroD1
  • neurogenic differentiation 1
  • neurogenic differentiation factor 1
  • neurogenic helix-loop-helix protein NEUROD
  • NIDDM

Background

This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NeuroD1 Antibody (H00004760-M01)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: IF/IHC.


Filter By Application
IF/IHC
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species
Showing Publications 1 - 3 of 3.
Publications using H00004760-M01 Applications Species
Wang Y, Hersheson J, Lopez D et al. Defects in the CAPN1 Gene Result in Alterations in Cerebellar Development and Cerebellar Ataxia in Mice and Humans. Cell Rep 2016-06-28 [PMID: 27320912] (IF/IHC, Mouse) IF/IHC Mouse
Akazawa M, Kawachi H, Kitagaki K et al. Neuroendocrine carcinoma of the esophagus: clinicopathologic study of 10 cases and verification of the diagnostic utility of mASH1, NeuroD1, and PGP9.5. Esophagus 2014-06-13 (IF/IHC, Human) IF/IHC Human
Chteinberg E, Sauer CM, Rennspiess D et al. Neuroendocrine Key Regulator Gene Expression in Merkel Cell Carcinoma. Neoplasia 2018-11-07 [PMID: 30414538]

Reviews for NeuroD1 Antibody (H00004760-M01) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NeuroD1 Antibody (H00004760-M01) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NEUROD1
Entrez
OMIM
Uniprot