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NASP Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: NASP Antibody [NBP2-33928] - Staining in human testis and pancreas tissues using anti-NASP antibody. Corresponding NASP RNA-seq data are presented for the ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: NASP Antibody [NBP2-33928] - Staining of human liver, lymph node, pancreas and testis using Anti-NASP antibody NBP2-33928 (A) shows similar protein ...read more
Genetic Strategies: Western Blot: NASP Antibody [NBP2-33928] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-NASP antibody. Remaining relative intensity ...read more
Western Blot: NASP Antibody [NBP2-33928] - Analysis in control (vector only transfected HEK293T lysate) and NASP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: NASP Antibody [NBP2-33928] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: NASP Antibody [NBP2-33928] - Staining of human lymph node.
Immunohistochemistry-Paraffin: NASP Antibody [NBP2-33928] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: NASP Antibody [NBP2-33928] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: NASP Antibody [NBP2-33928] - Staining of human liver.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NASP Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: KESQRSGNVAELALKATLVESSTSGFTPGGGGSSVSMIASRKPTDGASSSNCVTDISHLVRKKRKPEEESPRKDDAKKAKQEPEVNGGSGDAVPSG
Predicted Species
Mouse (96%), Rat (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NASP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NASP Protein (NBP2-33928PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NASP Antibody

  • DKFZp547F162
  • FLB7527
  • FLJ31599
  • FLJ35510
  • histone H1-binding protein
  • MGC19722
  • MGC20372
  • MGC2297
  • nuclear autoantigenic sperm protein (histone-binding)
  • nuclear autoantigenic sperm protein
  • PRO1999

Background

NASP is required for DNA replication, normal cell cycle progression, and cell proliferation. It forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA. [from: http://www.genecards.org/cgi-bin/carddisp.pl®gene=NASP&search=NASP]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NASP
Uniprot