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NARS Antibody

Images

 
Western Blot: NARS Antibody [NBP2-13640] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: NARS Antibody [NBP2-13640] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: NARS Antibody [NBP2-13640] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
Western Blot: NARS Antibody [NBP2-13640] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: NARS Antibody [NBP2-13640] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: NARS Antibody [NBP2-13640] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: NARS Antibody [NBP2-13640] - Staining of human liver shows very weak cytoplasmic positivity in hepatocytes.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NARS Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: RLEDLVCDVVDRILKSPAGSIVHELNPNFQPPKRPFKRMNYSDAIVWLKEHDVKKEDGTFYEFGEDIPEAPERLMTDTINEPILLCRFPVEIK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NARS1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,/Permeabilization: PFA/Triton X-100.
Control Peptide
NARS Protein (NBP2-13640PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NARS Antibody

  • ASNRS
  • asparagine tRNA ligase 1, cytoplasmic
  • Asparagine--tRNA ligase
  • asparaginyl-tRNA synthetase
  • asparaginyl-tRNA synthetase, cytoplasmic
  • EC 6.1.1.22
  • NARS1

Background

NARS, aminoacyl-tRNA synthetases, are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NARS Antibody (NBP2-13640) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NARS1