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DARS2 Antibody

Images

 
Independent Antibodies: Immunohistochemistry-Paraffin: DARS2 Antibody [NBP1-83854] - Staining of human colon, kidney, liver and lymph node using Anti-DARS2 antibody NBP1-83854 (A) shows similar protein ...read more
Independent Antibodies: Western Blot: DARS2 Antibody [NBP1-83854] - Analysis using Anti-DARS2 antibody NBP1-83854 (A) shows similar pattern to independent antibody NBP1-83855 (B).
Immunocytochemistry/ Immunofluorescence: DARS2 Antibody [NBP1-83854] - Staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.
Immunohistochemistry-Paraffin: DARS2 Antibody [NBP1-83854] - Staining of human lymph node.
Immunohistochemistry-Paraffin: DARS2 Antibody [NBP1-83854] - Staining of human kidney.
Immunohistochemistry-Paraffin: DARS2 Antibody [NBP1-83854] - Staining of human liver.
Immunohistochemistry-Paraffin: DARS2 Antibody [NBP1-83854] - Staining of human colon.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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DARS2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LSKPHGTVKAICIPEGAKYLKRKDIESIRNFAADHFNQEILPVFLNANRNWNSPVANFIMESQRLELIRLMETQEEDVVLLTA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DARS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DARS2 Protein (NBP1-83854PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DARS2 Antibody

  • aspartate tRNA ligase 2, mitochondrial
  • Aspartate--tRNA ligase
  • aspartyl-tRNA synthetase 2, mitochondrial
  • aspartyl-tRNA synthetase, mitochondrial
  • AspRS
  • ASPRS. LBSL
  • EC 6.1.1
  • EC 6.1.1.12
  • FLJ10514
  • LBSL
  • MT-ASPRS
  • RP3-383J4.2

Background

The protein encoded by the DARS2 gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DARS2 Antibody (NBP1-83854) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DARS2