Immunocytochemistry/ Immunofluorescence: MyoD Antibody [NBP1-54153] - RD cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated ...read more
Western Blot: MyoD1 Antibody [NBP1-54153] - Detection of MYOD1 in RH30 cells.
Immunocytochemistry/ Immunofluorescence: MyoD Antibody [NBP1-54153] - Staing of MYOD1 in RD cells with FITC (green). Nuclei were counterstained with DAPI (blue).
MyoD was detected in immersion fixed RD human Rhabdomyosarcoma cell line with Rabbit anti-MyoD Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP1-54153AF647) (light blue) at 5 ...read more
Mouse reactivity reported in scientific literature (PMID: 28569745).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified
Notes
Manufactured by Genomic Antibody Technology™. GAT FAQs
Alternate Names for MyoD Antibody - BSA Free
bHLHc1
bHLHc1BHLHC1
Class C basic helix-loop-helix protein 1
MYF3
MYF3Myf-3
MyoD
MYOD1
MYODmyoblast determination protein 1
myogenic differentiation 1
Myogenic factor 3myf-3
PUM
Background
MyoD1 is a myogenic bHLH transcription factor which is implicated in muscle differentiation via activation of muscle-specific promoters and is considered a master regulator of skeletal myogenesis as its expression induces myogenic differentiation in myoblasts, fibroblasts, and certain other cell types. MyoD1 is expressed during muscle development and remains silent in most cell types, and is associated with modified histones targeted to repressed genes. MyoD1 primers have been designed to amplify DNA in exon 1 of human MyoD1 gene locus and these primer sets are supplied premixed with forward and reverse primers in 10 uM in TE buffer. MyoD1 primers are compatible with all real-time PCR reactions and each primer set has been test validated by ChIP.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Corbel S, Sampath S, Schmedt C, Zhang Q COMPOSITIONS, METHODS, AND THERAPEUTIC USES RELATED TO FUSOGENIC PROTEIN MINION United States Patent Application 20190263899 2019-08-29
Jin Z, Da W, Huang C et al. Synergistic effects of herbal compounds to promote osteoporotic fracture repair through upregulation of B-catenin signaling in skeletal muscle satellite cells Authorea 2022-01-01 (IHC-P, WB, Mouse)
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