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Human MYF-5 - Ready-To-Use ELISA Kit (Colorimetric)

Name: Human MYF-5 - Ready-To-Use ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-39528

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ELISA: Human MYF-5 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39528] - Standard Curve Reference

Product Details

Summary

Reactivity	HuSpecies Glossary
Applications	ELISA
Suitable Sample Type	Tissue homogenates and other biological fluids
Standard Curve Range	0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity	0.066 ng/mL (example only; lot dependent)

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Human MYF-5 - Ready-To-Use ELISA Kit (Colorimetric) Summary

Description	The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time. Assay Length: 3 hours
Standard Curve Range	0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity	0.066 ng/mL (example only; lot dependent)
Assay Type	Sandwich ELISA
Inter-Assay	%CV < 12 (example only; lot dependent)
Intra-Assay	%CV < 10 (example only; lot dependent)
Sample Volume	100 uL
Kit Type	ELISA Kit (Colorimetric)
Gene	MYF5

Applications/Dilutions

Dilutions

ELISA

Packaging, Storage & Formulations

Storage

Storage of components varies. See protocol for specific instructions.

Kit Components

Components

Detection Solution A
Detection Solution B
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard Diluent
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Alternate Names for Human MYF-5 - Ready-To-Use ELISA Kit (Colorimetric)

bHLHc2
bHLHc2Class C basic helix-loop-helix protein 2
MYF5
MYF-5
myogenic factor 5

Background

nvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Human MYF-5 -
Ready-To-Use ELISA Ki ...

NBP3-39528

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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Myosin Heavy Chain was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Myosin Heavy Chain Antibody (MF20) [Unconjugated]
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Bioinformatics

Gene Symbol

MYF5

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Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Human MYF-5 - Ready-To-Use ELISA Kit (Colorimetric)

Species Glossary

Images

Product Details

Order Details

Human MYF-5 - Ready-To-Use ELISA Kit (Colorimetric) Summary

Applications/Dilutions

Packaging, Storage & Formulations

Kit Components

Alternate Names for Human MYF-5 - Ready-To-Use ELISA Kit (Colorimetric)

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for MYF-5 ELISA Kit (NBP3-39528) (0)

Reviews for MYF-5 ELISA Kit (NBP3-39528) (0)

Product General Protocols

FAQs for MYF-5 ELISA Kit (NBP3-39528) (0)

Additional MYF-5 Products

Blogs on MYF-5.

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Contact Information

Product PDFs

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Bioinformatics