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MYF-5 Antibody

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Western Blot: MYF-5 Antibody [NB100-93518] - (0.5ug/ml) staining of Muscle lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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MYF-5 Antibody Summary

Immunogen
Peptide with sequence PECNSPVWSRKSST corresponding to internal region according to NP_005584.1.
Epitope
PECNSPVWSRKSST
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MYF5
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 1.5 ug/ml
Application Notes
WB: Approx. 28 kDa band observed in human skeletal muscle lysates (calculated MW of 28.4 kDa band according to NP_005584.1). An additional band of 26 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than WB.
Publications
Read Publication using NB100-93518.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MYF-5 Antibody

  • bHLHc2
  • bHLHc2Class C basic helix-loop-helix protein 2
  • MYF5
  • MYF-5
  • myogenic factor 5

Background

nvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MYF-5 Antibody (NB100-93518)(1)


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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol MYF5
Entrez
Uniprot