MUC2 Antibody

MUC2, also called mucin 2, is a member of the mucin protein family which are heavily glycosylated, high molecular weight proteins that are produced by goblet cells in epithelial tissues including the intestinal and respiratory tract (1). Mucins can form gels and function in lubrication of mucosal surfaces which serve as a protective barrier of the epithelial lining and an innate host defense (1). Mucins are divided into two groups based on whether they are membrane-bound or secreted, where MUC2 belongs to the secretory group (1,2). Secreted mucins have a distinctive composition characterized by a large central exon "mucin domain" containing O-glycosylated tandem repeat regions rich in proline, threonine, and serine residues, non-repetitive domains, and cysteine-rich domains (1,2). The mucin domain is flanked by 5' and 3' D domains, a B-like domain, a C-like domain, and a Ck-domain (1,2). MUC2 was the first secretory mucin identified in humans (3,4). Full-length MUC2 protein is synthesized as 5179 amino acids (aa) in length with a theoretical molecular weight of ~540 kDa (5).

Changes or perturbations to MUC2 expression has been associated with a number of disease pathologies (1-4, 6). Specifically, altered MUC2 composition has been indicated in colorectal cancer, inflammatory bowel diseases (IBD) including ulcerative colitis and Chron's disease, and chronic obstructive pulmonary disease (COPD) (1-4, 6). In general, decreased or reduced MUC2 expression is associated with colorectal cancer disease progression and development of IBD (1-4, 6). Additionally, studies have found that upon intestinal infection due to parasites or bacteria MUC2 expression is increased, as is overall mucus secretion (3, 6).

References

1. Pothuraju, R., Krishn, S. R., Gautam, S. K., Pai, P., Ganguly, K., Chaudhary, S., Rachagani, S., Kaur, S., & Batra, S. K. (2020). Mechanistic and Functional Shades of Mucins and Associated Glycans in Colon Cancer. Cancers. https://doi.org/10.3390/cancers12030649

2. Ballester, B., Milara, J., & Cortijo, J. (2019). Mucins as a New Frontier in Pulmonary Fibrosis. Journal of Clinical Medicine. https://doi.org/10.3390/jcm8091447

3. Liu, Y., Yu, X., Zhao, J., Zhang, H., Zhai, Q., & Chen, W. (2020). The role of MUC2 mucin in intestinal homeostasis and the impact of dietary components on MUC2 expression. International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2020.07.191

4. Allen, A., Hutton, D. A., & Pearson, J. P. (1998). The MUC2 gene product: a human intestinal mucin. The International Journal of Biochemistry & Cell Biology. https://doi.org/10.1016/s1357-2725(98)00028-4

5. Uniprot (Q02817)

6. Kim, Y. S., & Ho, S. B. (2010). Intestinal goblet cells and mucins in health and disease: recent insights and progress. Current Gastroenterology Reports. https://doi.org/10.1007/s11894-010-0131-2

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: Cytometric Bead Assay Standard, Dot Blot, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, WB.

Showing Publications 1 - 10 of 38. Show All 38 Publications. Collapse Publications.

Publications using NBP1-31231	Applications	Species
Judith C. W. de Jong, Kristel S. van Rooijen, Edwin C. A. Stigter, M. Can Gülersönmez, Marcel R. de Zoete, Janetta Top, Matthijs J. D. Baars, Yvonne Vercoulen, Folkert Kuipers, Saskia W. C. van Mil, Noortje Ijssennagger, Birendra Mishra Dietary cystine restriction increases the proliferative capacity of the small intestine of mice PLOS ONE 2024-01-05 [PMID: 38181033]
HAfliger J, Schwarzfischer M, Atrott K et al. Glycoprotein (GP)96 Is Essential for Maintaining Intestinal Epithelial Architecture by Supporting Its Self-Renewal Capacity Cellular and molecular gastroenterology and hepatology 2022-12-11 [PMID: 36516930] (IHC-P, Mouse)	IHC-P	Mouse
Luck H The Role of the Intestinal Immune System in Diet-induced Obesity and Insulin Resistance Thesis 2020-01-01 (ICC/IF, Mouse)	ICC/IF	Mouse
Chamignon C, Mallaret G, Rivi�re J et al. Beneficial Effects of Lactobacilli Species on Intestinal Homeostasis in Low-Grade Inflammation and Stress Rodent Models and Their Implication in the Modulation of the Adhesive Junctional Complex Biomolecules 2023-08-24 [PMID: 37759696]
Efren A. Reyes, David Castillo-Azofeifa, Jérémie Rispal, Tomas Wald, Rachel K. Zwick, Brisa Palikuqi, Angela Mujukian, Shervin Rabizadeh, Alexander R. Gupta, James M. Gardner, Dario Boffelli, Zev J. Gartner, Ophir D. Klein Epithelial TNF controls cell differentiation and CFTR activity to maintain intestinal mucin homeostasis The Journal of Clinical Investigation 2023-10-16 [PMID: 37643009]
Sonomoto K, Song R, Eriksson D et al. High-fat-diet-associated intestinal microbiota exacerbates psoriasis-like inflammation by enhancing systemic ?? T�cell IL-17 production Cell Reports 2023-07-25 [PMID: 37421628] (ICC/IF)	ICC/IF
Chae SA, Du M, Son JS, Zhu MJ Exercise improves homeostasis of the intestinal epithelium by activation of apelin receptor-AMP-activated protein kinase signalling The Journal of physiology 2023-05-08 [PMID: 37154385]
Oosterlinck B, Ceuleers H, Arras W et al. Mucin-microbiome signatures shape the tumor microenvironment in gastric cancer Microbiome 2023-04-21 [PMID: 37085819]
Castillo-Azofeifa D, Wald T, Reyes EA et al. A DLG1-ARHGAP31-CDC42 axis is essential for the intestinal stem cell response to fluctuating niche Wnt signaling Cell stem cell 2023-01-05 [PMID: 36640764] (IHC-Fr, IHC-P, Mouse)	IHC-Fr, IHC-P	Mouse
Breugelmans T, Arras W, Boen LE et al. Aberrant Mucin Expression Profiles Associate With Pediatric Inflammatory Bowel Disease Presentation and Activity Inflammatory bowel diseases 2022-10-14 [PMID: 36239641]
Chae SA, Son JS, de Avila JM et al. Maternal exercise improves epithelial development of fetal intestine by enhancing apelin signaling and oxidative metabolism American journal of physiology. Regulatory, integrative and comparative physiology 2022-11-01 [PMID: 36189989] (IHC-P, WB, Mouse) Details: Dilution used for IHC 1:50, for WB 1:1000	IHC-P, WB	Mouse
Palikuqi B, Rispal J, Reyes EA et al. Lymphangiocrine signals are required for proper intestinal repair after cytotoxic injury Cell stem cell 2022-08-04 [PMID: 35931034] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Lacroix G, Gouyer V, Rocher M et al. A porous cervical mucus plug leads to preterm birth induced by experimental vaginal infection in mice iScience 2022-07-15 [PMID: 35754724] (Mouse)		Mouse
Das TK, Blasco-Conesa MP, Korf J et al. Bacterial Amyloid Curli Associated Gut Epithelial Neuroendocrine Activation Predominantly Observed in Alzheimer's Disease Mice with Central Amyloid-? Pathology Journal of Alzheimer's Disease 2022-08-30 [PMID: 35527554] (IHC, WB)	IHC, WB
Zangara, M;Ponti, A;Miller, N;Engelhart, M;Ahern, P;Sangwan, N;McDonald, C; Maltodextrin Consumption Impairs the Intestinal Mucus Barrier and Accelerates Colitis Through Direct Actions on the Epithelium Frontiers in Immunology [PMID: 35359925] (ICC/IF, Mouse)	ICC/IF	Mouse
Higa, T;Okita, Y;Matsumoto, A;Nakayama, S;Oka, T;Sugahara, O;Koga, D;Takeishi, S;Nakatsumi, H;Hosen, N;Robine, S;Taketo, MM;Sato, T;Nakayama, KI; Spatiotemporal reprogramming of differentiated cells underlies regeneration and neoplasia in the intestinal epithelium Nature communications [PMID: 35314700] (ICC/IF, Mouse)	ICC/IF	Mouse
Melhem H, Kaya B, Kaymak T et al. Epithelial GPR35 protects from Citrobacter rodentium infection by preserving goblet cells and mucosal barrier integrity Mucosal Immunology 2022-03-09 [PMID: 35264769] (ICC/IF)	ICC/IF
Daniel Zeve, Eric Stas, Joshua de Sousa Casal, Prabhath Mannam, Wanshu Qi, Xiaolei Yin, Sarah Dubois, Manasvi S. Shah, Erin P. Syverson, Sophie Hafner, Jeffrey M. Karp, Diana L. Carlone, Jose Ordovas-Montanes, David T. Breault Robust differentiation of human enteroendocrine cells from intestinal stem cells Nature Communications 2022-01-11 [PMID: 35017529]
Noortje Ijssennagger, Kristel S. van Rooijen, Stefanía Magnúsdóttir, José M. Ramos Pittol, Ellen C.L. Willemsen, Marcel R. de Zoete, Matthijs J.D. Baars, Paul B. Stege, Carolina Colliva, Roberto Pellicciari, Sameh A. Youssef, Alain de Bruin, Yvonne Vercoulen, Folkert Kuipers, Saskia W.C. van Mil Ablation of liver Fxr results in an increased colonic mucus barrier in mice JHEP Reports 2021-08-04 [PMID: 34604725]
Kwon MS, Chung HK, Xiao L et al. MicroRNA-195 regulates Tuft cell function in the intestinal epithelium by altering translation of double cortin-like kinase 1 American journal of physiology. Cell physiology 2021-03-31 [PMID: 33788631]
Tomita H, Tanaka K, Hirata A et al. Inhibition of FGF10-ERK signal activation suppresses intraductal papillary neoplasm of the bile duct and its associated carcinomas Cell reports 2021-02-23 [PMID: 33626352]
Tirado FR, Bhanja P, Castro-Nallar E, et al. Radiation-induced toxicity in rectal epithelial stem cell contributes to acute radiation injury in rectum Stem cell research & therapy 2021-01-15 [PMID: 33451351] (ICC/IF, Mouse)	ICC/IF	Mouse
Rishi Drolia, Mary Anne Roshni Amalaradjou, Valerie Ryan, Shivendra Tenguria, Dongqi Liu, Xingjian Bai, Luping Xu, Atul K. Singh, Abigail D. Cox, Victor Bernal-Crespo, James A. Schaber, Bruce M. Applegate, Ramesh Vemulapalli, Arun K. Bhunia Receptor-targeted engineered probiotics mitigate lethal Listeria infection Nature Communications 2020-12-11 [PMID: 33311493]
Engevik M, Danhof HA, Auchtung J et al. Fusobacterium nucleatum adheres to Clostridioides difficile via the RadD adhesin to enhance biofilm formation in intestinal mucus Gastroenterology 2020-11-20 [PMID: 33227279]
Shastri S, Shinde T, Perera AP et al. Idebenone Protects against Spontaneous Chronic Murine Colitis by Alleviating Endoplasmic Reticulum Stress and Inflammatory Response Biomedicines 2020-09-28 [PMID: 32998266]
Zeng B, Wang D, et al. Dietary Soy Protein Isolate Attenuates Intestinal Immunoglobulin and Mucin Expression in Young Mice Compared with Casein. Nutrients 2020-09-08 [PMID: 32911830] (IF/IHC, Mouse)	IF/IHC	Mouse
Yan J, Wang D, Li K et al. Toxic effects of the food additives titanium dioxide and silica on the murine intestinal tract: Mechanisms related to intestinal barrier dysfunction involved by gut microbiota Environmental toxicology and pharmacology 2020-09-03 [PMID: 32891757] (IHC-P, Mouse)	IHC-P	Mouse
Engevik MA, Danhof HA, Shrestha R et al. Reuterin disrupts Clostridioides difficile metabolism and pathogenicity through reactive oxygen species generation Gut Microbes 2020-11-09 [PMID: 32804011] (IF/IHC, Human)	IF/IHC	Human
Engevik MA, Danhof HA, Chang-Graham AL et al. Human intestinal enteroids as a model of Clostridioides difficile-induced enteritis Am. J. Physiol. Gastrointest. Liver Physiol. 2020-05-01 [PMID: 32223302] (Cytometric Bead Assay Standard)	Cytometric Bead Assay Standard
Schwerdtfeger LA, Tobet SA Vasoactive intestinal peptide regulates ileal goblet cell production in mice Physiol Rep 2020-02-01 [PMID: 32026594] (IF/IHC, Mouse)	IF/IHC	Mouse
Seo B, Jeon K, Moon S, et al. Roseburia spp. Abundance Associates with Alcohol Consumption in Humans and Its Administration Ameliorates Alcoholic Fatty Liver in Mice Cell Host Microbe 2019-12-19 [PMID: 31866426] (Mouse)		Mouse
Miyake H, Lee C, Chusilp S et al. Human breast milk exosomes attenuate intestinal damage Pediatr. Surg. Int. 2019-11-12 [PMID: 31713717]
Li B, Hock A, Wu RY et al. Bovine milk-derived exosomes enhance goblet cell activity and prevent the development of experimental necrotizing enterocolitis PLoS ONE 2019-01-30 [PMID: 30699187] (ICC/IF, Human)	ICC/IF	Human
Janardhan KS, Jensen H, Clayton NP, Herbert RA Immunohistochemistry in Investigative and Toxicologic Pathology Toxicol Pathol. 2018-07-01 [PMID: 29966501] (IF/IHC, IF/IHC, Mouse) Details: Citation used the HRP format of this antibody.	IF/IHC, IF/IHC	Mouse
Yasuda-Onozawa Y, Handa O et al. Rebamipide upregulates mucin secretion of intestinal goblet cells via Akt phosphorylation. Mol Med Rep 2017-01-12 [PMID: 28983630] (Dot Blot, Human)	Dot Blot	Human
Kameoka Y, Kitazawa R, Ariasu K et al. Reactivation of CDX2 in Gastric Cancer as Mark for Gene Silencing Memory. Acta Histochem Cytochem 2015-08-27 [PMID: 26379313] (IHC-P, Human)	IHC-P	Human
de Jong J, van Rooijen K, Stigter E et al. Dietary cystine restriction increases the proliferative capacity of the small intestine of mice bioRxiv 2023-08-12 (IHC, Mouse)	IHC	Mouse
Breugelmans T, Van Spaendonk H, De Man JG et al. In depth study of transmembrane mucins in association with intestinal barrier dysfunction during the course of T cell transfer and DSS-induced colitis J Crohns Colitis 2020-01-31 [PMID: 32003421]
Show All 38 Publications. Collapse Publications.

Array NBP1-31231

• MUC2 Antibodies
• MUC2 ELISA Kits