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MUC4 Antibody (5B12)

Images

 
Western Blot: MUC4 Antibody (5B12) [H00004585-M07] - Analysis of MUC4 expression in HeLa (Cat # L013V1).
Immunohistochemistry-Paraffin: MUC4 Antibody (5B12) [H00004585-M07] - Analysis of monoclonal antibody to MUC4 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml
ELISA: MUC4 Antibody (5B12) [H00004585-M07] - Detection limit for recombinant GST tagged MUC4 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC
Clone
5B12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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MUC4 Antibody (5B12) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
MUC4 (NP_004523, 79 a.a. ~ 188 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GVSLFPYGADAGDLEFVRRTVDFTSPLFKPATGFPLGSSLRDSLYFTDNGQIIFPESDYQIFSYPNPLPTGFTGRDPVALVAPFWDDADFSTGRGTTFYQEYETFYGEHS
Specificity
MUC4 - mucin 4, tracheobronchial
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MUC4
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Publications
Read Publications using H00004585-M07.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MUC4 Antibody (5B12)

  • Ascites sialoglycoprotein
  • ASGP
  • HSA276359
  • MUC4
  • MUC-4
  • mucin 4, cell surface associated
  • mucin 4, tracheobronchial
  • mucin-4
  • Pancreatic adenocarcinoma mucin
  • Testis mucin
  • Tracheobronchial mucin

Background

The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. This gene contains a region in the coding sequence which has a variable number (>100) of 48 nt tandem repeats. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, IHC

Publications for MUC4 Antibody (H00004585-M07)(5)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MUC4 Antibody (H00004585-M07). (Showing 1 - 1 of 1 FAQs).

  1. I am particularly interested in your mucin 4 5B12 clone. On your website you state that you can conjugate many of your primary antibodies with biotin. Would I be able to custom order this antibody biotinylated? What would the cost/minimum order volume be?
    • The 5B12 clone we could custom conjugate to biotin for you for a flat rate of $80 USD. This would be for the 0.1mg size the antibody is supplied in. We cannot guarantee the yield following conjugation and do not perform additional QC.

Secondary Antibodies

 

Isotype Controls

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Research Areas for MUC4 Antibody (H00004585-M07)

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Blogs on MUC4.

MUC4 (Mucin-4)
Mucus is the viscous secretion that covers epithelial surfaces (trachea, colon, and cervix) and consists of twenty highly glycosylated proteins called mucins. The mucin family all are high-molecular weight proteins with oligosaccharides attached to th...  Read full blog post.

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Bioinformatics

Gene Symbol MUC4
Entrez
OMIM
Uniprot