Immunohistochemistry-Paraffin: MUC6 Antibody (CLH5) [NBP2-44374] - Formalin-fixed, paraffin-embedded human normal stomach. Strong cytoplasmic of glandular cells using MUC6 Antibody (CLH5). HIER: Tris/EDTA, pH9.0, 45min. ...read more
Immunohistochemistry-Paraffin: MUC6 Antibody (CLH5) [NBP2-44374] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC6 Antibody (CLH5). Courtesy of Dr. Leonor David, IPATIMUP and Medical Faculty, ...read more
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11450)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
A synthetic peptide of the Gastric MUC6 tandem repeat sequence (Uniprot: Q6W4X9 )
Localization
Cytoplasmic & Secreted
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MUC6
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Theoretical MW
252 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NBP2-44374 in the following applications:
Mucin is a high M.W. (>1,000 kDa) glycoprotein, expressed by mucus cells of the gastric epithelium and by goblet cells of the fetal, precancerous and cancerous colon, but not by those of the normal colon. It also appears in other epithelial tissues, which are embryologically derived from the foregut (epigastric and bronchial epithelium) and in M ller ducts (mucus cells of the endocervix and urethral epithelium near the prostatic utriculus).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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