![Western Blot: MTHFR Antibody [NBP3-17040] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/MTHFR-Antibody-Western-Blot-NBP3-17040-img0006.jpg "Western Blot: MTHFR Antibody [NBP3-17040] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to the amino acids: TILHMTCCRQRLEEITGHLHKAKQLGLKNIMALRGDPIGDQWEEEEGGFNYAVDLVKHIRSEFGDYFDICVAGYPKGHPEAGS |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MTHFR |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.2, 40% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Immunohistochemistry ACE/CD143 Antibody [Unconjugated]](https://images.novusbio.com/images2/ACE_AF1513_Immunohistochemistry_6703.jpg)
![Western Blot ACE/CD143 Antibody [Unconjugated]](https://images.novusbio.com/images2/ACE_AF1513_Flow_Cytometry_20028.jpg)
![Simple Western ACE/CD143 Antibody [Unconjugated]](https://images.novusbio.com/images2/ACE_AF1513_Simple_Western_20109.jpg)
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MTHFR |
![Immunohistochemistry-Paraffin: MTHFR Antibody [NBP3-17040] - Staining of human pancrease shows low expression as expected.](http://images.novusbio.com/fullsize/MTHFR-Antibody-Immunohistochemistry-Paraffin-NBP3-17040-img0004.jpg "Immunohistochemistry-Paraffin: MTHFR Antibody [NBP3-17040] - Staining of human pancrease shows low expression as expected.")
![Immunohistochemistry-Paraffin: MTHFR Antibody [NBP3-17040] - Analysis in human epididymis and pancreas tissues using Anti-MTHFR antibody. Corresponding MTHFR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MTHFR-Antibody-Immunohistochemistry-Paraffin-NBP3-17040-img0001.jpg "Immunohistochemistry-Paraffin: MTHFR Antibody [NBP3-17040] - Analysis in human epididymis and pancreas tissues using Anti-MTHFR antibody. Corresponding MTHFR RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: MTHFR Antibody [NBP3-17040] - Staining of human epididymis shows hight expression.](http://images.novusbio.com/fullsize/MTHFR-Antibody-Immunohistochemistry-Paraffin-NBP3-17040-img0002.jpg "Immunohistochemistry-Paraffin: MTHFR Antibody [NBP3-17040] - Staining of human epididymis shows hight expression.")
![Immunohistochemistry-Paraffin: MTHFR Antibody [NBP3-17040] - Staining of human piuitary gland shows strong positivity in cells in anterior.](http://images.novusbio.com/fullsize/MTHFR-Antibody-Immunohistochemistry-Paraffin-NBP3-17040-img0003.jpg "Immunohistochemistry-Paraffin: MTHFR Antibody [NBP3-17040] - Staining of human piuitary gland shows strong positivity in cells in anterior.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")