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MSP/MST1 Antibody

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Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse MSP/MST1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6244) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MSP/MST1 at approximately 96 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse MSP/MST1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6244) followed by HRP-conjugated ...read more
MSP/MST1 was detected in perfusion fixed frozen sections of mouse liver using Sheep Anti-Mouse MSP/MST1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6244) at 15 µg/mL overnight at 4 °C. Tissue was ...read more
MSP stimulates AKT and MAPK signaling pathways through the RON receptor. (A) Mst1r mRNA expression assessed by qRT–PCR in cell lines derived from three KP tumors and four KB1P tumors, respectively. (B) Western blot ...read more
MSP and RON are upregulated in tumors from KB1P mice. (A) Mst1 and Mst1r mRNA expression in normal MG (from three individual mice), KP tumors (n = 44) and KB1P tumors (n = 41) analyzed by RNA sequencing. Boxplots ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF6244-SP
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MSP/MST1 Antibody Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse MSP/MST1
Gly19-Gly716 (Cys677Ala)
Accession # NP_032269
Specificity
Detects mouse MSP/MST1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human MSP is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
MST1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using AF6244.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MSP/MST1 Antibody

  • D3F15S2
  • EC 3.4.21
  • hepatocyte growth factor-like protein homolog
  • HGFL
  • HGFLhepatocyte growth factor-like protein
  • macrophage stimulating 1 (hepatocyte growth factor-like)
  • Macrophage stimulatory protein
  • Macrophage-stimulating protein
  • MSP
  • MSPDNF15S2
  • MST1
  • NF15S2
  • SF2

Background

MSP (Macrophage stimulating protein 1; also hepatocyte growth factor-like protein) is an 80-95 kDa member of the peptidase S1 family of proteins. Although it is expressed principally by hepatocytes, it can also be induced in cells such as renal tubular epithelium. MSP has multiple targets, and as such, has multiple effects. It stimulates macrophage motility and phagocytosis, promotes keratinocyte and renal tubular cell proliferation, and depresses myeloid progenitor cell replication. Mouse proMSP is 698 amino acids (aa) in length. It contains one PAN (carbohydrate-binding) site (aa 19-105), four consecutive kringle domains (110-457) and an inactive peptidase S1 region (aa 489-714). An intrachain disulfide bond exists between Cys477 and Cys593 that becomes an interchain bond following protease cleavage between Arg488 and Val489. This creates a mature heterodimer containing a 45-57 kDa alpha -chain, and a 30-35 kDa beta -chain. Mouse proMSP shares 80% and 93% aa identity with human and rat proMSP, respectively.

                        

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MSP/MST1 Antibody (AF6244)(1)


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Bioinformatics

Gene Symbol MST1
Uniprot