Home » MSPR/Ron » MSPR/Ron Antibodies » MSPR/Ron [p Tyr1238, p Tyr1239, p Tyr1239] Antibody [Unconjugated]
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MSPR/Ron [p Tyr1238, p Tyr1239, p Tyr1239] Antibody [Unconjugated]

 (12 Publications)
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Western blot shows lysates of MDA-MB-453 human breast cancer cell line untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. The membranes were untreated or treated with calf intestinal phosphatase (CIP). PVDF membrane was probed with 1 µg/mL of Human Phospho-MSP R/Ron (Y1238/Y1239) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MSP R/Ron (Y1238/Y1239) at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of MDA-MB-453 human breast cancer cell line untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. The membranes were untreated or treated with calf intestinal phosphatase ...read more
Human Phospho-MSP R/Ron (Y1238/Y1239) was immunoprecipitated from 1 mg of lysates of MDA‑MB‑453 human breast cancer cell line untreated (-) or treated (+) with 100 uM Pervanadate (PV) for 10 minutes or 100 ng/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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AF1947-SP
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MSPR/Ron [p Tyr1238, p Tyr1239, p Tyr1239] Antibody [Unconjugated] Summary

Immunogen
Phosphopeptide containing human MSP R/Ron Y1238/Y1239 sites
Modification
p Tyr1239, p Tyr1238, p Tyr1239
Specificity
The antibody detects endogenous human MSP R/Ron phosphorylated at Y1238/Y1239 using Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MST1R
Purity Statement
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF1947 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MSPR/Ron [p Tyr1238, p Tyr1239, p Tyr1239] Antibody [Unconjugated]

  • CD136 antigen
  • CD136
  • CDw136c-met-related tyrosine kinase
  • EC 2.7.10
  • EC 2.7.10.1
  • macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
  • MSP R
  • MSP receptor
  • MSPR
  • MST1R
  • p185-Ron
  • Protein-tyrosine kinase 8
  • PTK8 protein tyrosine kinase 8
  • PTK8
  • Ron
  • RONmacrophage-stimulating protein receptor
  • soluble RON variant 1
  • soluble RON variant 2
  • soluble RON variant 3
  • soluble RON variant 4

Background

Macrophage stimulating protein receptor (MSP R), also known as Ron, is one of a small family of receptor tyrosine kinases (RTKs) that also includes human Met and chicken Sea. Each family member is synthesized as a single-chain precursor that is cleaved into a mature disulfide-linked heterodimer composed of an extracellular alpha  chain and a membrane-spanning beta  chain with intrinsic tyrosine kinase activity. MSP R/Ron is overexpressed in a number of primary breast carcinomas and non-small cell lung carcinomas, suggesting a role for this RTK in mammary and lung tumor progression. Phosphorylation of MSP R/Ron at Y1238/Y1239 in the activation loop of the kinase domain is responsible for kinase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MSPR/Ron [p
Tyr1238, p Tyr1239, p ...
AF1947
MSPR/Ron [p Tyr1238, p Tyr1239, p Tyr1239] Antibody [Unconjugated]
Species: Hu
Applications: WB

Publications for MSPR/Ron Antibody (AF1947)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC, IHC, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(1)
IHC
(1)
Immunoprecipitation
(1)
Western Blot
(4)
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Human
(6)
Mouse
(1)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF1947 Applications Species
Lindsay M. Gurska, Rachel Okabe, Alexandra Schurer, Meng Maxine Tong, Mark Soto, Daniel Choi et al. Crizotinib Has Preclinical Efficacy in Philadelphia-Negative Myeloproliferative Neoplasms Clinical Cancer Research 2023-03-01 [PMID: 36537918]
DM Beauvais, SE Nelson, KM Adams, NA Stueven, O Jung, AC Rapraeger Plasma membrane proteoglycans syndecan-2 and syndecan-4 engage with EGFR and RON kinase to sustain carcinoma cell cycle progression The Journal of Biological Chemistry, 2022-05-13;0(0):102029. 2022-05-13 [PMID: 35569509] (Immunoprecipitation, Human) Immunoprecipitation Human
Carolina Franco Nitta, Ellen W Green, Elton D Jhamba, Justine M Keth, Iraís Ortiz-Caraveo, Rachel M Grattan et al. EGFR transactivates RON to drive oncogenic crosstalk eLife 2021-11-25 [PMID: 34821550]
Q Yu, J Wang, T Li, X Guo, S Ding, X Che, L Zhu, Y Peng, X Xu, G Zou, X Zhang Recepteur d'origine nantais contributes to the development of endometriosis via promoting epithelial-mesenchymal transition of a endometrial epithelial cells Journal of Cellular and Molecular Medicine, 2021-01-06;0(0):. 2021-01-06 [PMID: 33410267] (Western Blot, Human) Western Blot Human
D Dustin, G Gu, AR Beyer, SK Herzog, DG Edwards, H Lin, TL Gonzalez, SL Grimm, C Coarfa, DW Chan, BJ Kim, JP De La O, MJ Ellis, D Liu, S Li, AL Welm, SAW Fuqua RON signalling promotes therapeutic resistance in ESR1 mutant breast cancer British Journal of Cancer, 2020-12-01;0(0):. 2020-12-01 [PMID: 33257837] (Western Blot, Human) Western Blot Human
P Berning, C Hennemann, C Tulotta, C Schaefer, B Lechtape, M Hotfilder, Y El Gourari, H Jürgens, E Snaar-Jaga, G Hempel, U Dirksen, J Potratz The Receptor Tyrosine Kinase RON and Its Isoforms as Therapeutic Targets in Ewing Sarcoma Cancers (Basel), 2020-04-07;12(4):. 4/7/2020 [PMID: 32272784]
M Balmaña, F Diniz, T Feijão, CC Barrias, S Mereiter, CA Reis Analysis of the Effect of Increased &alpha2,3-Sialylation on RTK Activation in MKN45 Gastric Cancer Spheroids Treated with Crizotinib Int J Mol Sci, 2020-01-22;21(3):. 2020-01-22 [PMID: 31979110] (ICC, Human) ICC Human
R Graves-Dea, G Bogatcheva, S Rehman, Y Lu, JN Higginboth, B Singh Broad-spectrum receptor tyrosine kinase inhibitors overcome de novo and acquired modes of resistance to EGFR-targeted therapies in colorectal cancer Oncotarget, 2019-02-12;10(13):1320-1333. 2019-02-12 [PMID: 30863492] (Western Blot, Human) Western Blot Human
Sasha J. Ruiz-Torres, Nancy M. Benight, Rebekah A. Karns, Elyse E. Lower, Jun-Lin Guan, Susan E. Waltz HGFL-mediated RON signaling supports breast cancer stem cell phenotypes via activation of non-canonical beta -catenin signaling Oncotarget 2017-08-29 [PMID: 28938607]
Jeffery Chakedis, Randall French, Michele Babicky, Dawn Jaquish, Evangeline Mose, Peter Cheng et al. Characterization of RON protein isoforms in pancreatic cancer: implications for biology and therapeutics Oncotarget 2016-07-19 [PMID: 27323855]
Show All 12 Publications.

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Bioinformatics

Gene Symbol MST1R