Novus Biologicals products are now on bio-techne.com

MSP/MST1 Antibody [Unconjugated]

Images

 
Recombinant Human MSP (Catalog # 352‑MS) chemoattracts mouse peritoneal resident macrophages in a dose-dependent manner (orange line). The amount of cells that migrated through the filter was measured by ...read more
TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF ...read more
TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF ...read more
TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

MSP/MST1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human MSP/MST1
Gln19-Gly711
Accession # AAA59872
Specificity
Detects human MSP/MST1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human HGF is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MST1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Neutralization 0.05-0.2 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF352 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MSP/MST1 Antibody [Unconjugated]

  • D3F15S2
  • EC 3.4.21
  • hepatocyte growth factor-like protein homolog
  • HGFL
  • HGFLhepatocyte growth factor-like protein
  • macrophage stimulating 1 (hepatocyte growth factor-like)
  • Macrophage stimulatory protein
  • Macrophage-stimulating protein
  • MSP
  • MSPDNF15S2
  • MST1
  • NF15S2
  • SF2

Background

Macrophage stimulating protein (MSP), also known as HGF-like protein, and scatter factor-2, is a member of the HGF family of growth factors (1). MSP is secreted as an inactive single chain precursor (pro-MSP) that contains a PAN/APPLE-like domain, four kringle domains, and a peptidase S1 domain which lacks enzymatic activity (2). Human MSP shares 79% aa sequence identity with mouse MSP and 44% aa sequence identity with human HGF. Pro-MSP is secreted by hepatocytes under the positive and negative control of CBP in complex with either HNF-4 or RAR, respectively (3). Circulating pro-MSP is proteolytically cleaved in response to tissue injury to yield biologically active disulfide linked heterodimers consisting of a 45‑62 kDa alpha and a 25‑35 kDa beta chain (4, 5). Pro-MSP can be activated by MT-SP1, a transmembrane protease that is expressed on macrophages and is upregulated in many cancers (6). Heterodimeric MSP as well as the isolated beta chain bind to MSP R/Ron with high-affinity, although only heterodimeric MSP can induce receptor dimerization and signaling (7, 8). MSP induces macrophage and keratinocyte proliferation and osteoclast activation (9, 10). It also inhibits LPS- or IFN-induced iNOS and IL-12 expression by macrophages and prevents apoptosis of epithelial cells separated from the ECM (11, 12).

  1. Wang, M.-H. et al. (2002) Scand. J. Immunol. 56:545.
  2. Han, S. et al. (1991) Biochemistry 30:9768.
  3. Muraoka, R.S. et al. (1999) Endocrinology 140:187.
  4. Wang, M.-H. et al. (1996) J. Clin. Invest. 97:720.
  5. Nanney, L.B. et al. (1998) J. Invest. Dermatol. 111:573.
  6. Bhatt, A.S. et al. (2007) Proc. Natl. Acad. Sci. 104:5771.
  7. Wang, M.-H. et al. (1997) J. Biol. Chem. 272:16999.
  8. Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937.
  9. Wang, M.-H. et al. (1996) Exp. Cell Res. 226:39.
  10. Kurihara, N. et al. (1998) Exp. Hematol. 26:1080.
  11. Morrison, A.C. et al. (2004) J. Immunol. 172:1825.
  12. Liu, Q.P. et al. (1999) J. Immunol. 163:6606.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB200-106
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
294-HG
Species: Hu
Applications: BA
NBP2-03644
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB100-168
Species: Hu, Mu(-)
Applications: CyTOF-ready, Flow-IC, Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
AF748
Species: Hu, Mu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, ICC, IHC, Simple Western, WB
NBP2-67381
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
NBP1-48017
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, WB
M6000B
Species: Mu
Applications: ELISA
NBP1-82454
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
H00006789-M01
Species: Hu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, PLA, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-45603
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
NB100-91662
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB100-56519
Species: Bv, Hu, Mu, Po, Rt, Sh
Applications: ChIP, CyTOF-ready, Flow-IC, Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, KD, Simple Western, WB
NBP2-16756
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NB200-323
Species: Hu, Rt
Applications: WB
AF2065
Species: Mu
Applications: ICC, IHC, Simple Western, WB
AF352
Species: Hu
Applications: WB, B/N

Publications for MSP/MST1 Antibody (AF352)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Co-Immunoprecipitation, Flow Cytometry, IHC-P, Neutralization, Western Blot.


Filter By Application
Co-Immunoprecipitation
(1)
Flow Cytometry
(1)
IHC-P
(1)
Neutralization
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species

Reviews for MSP/MST1 Antibody (AF352) (0)

There are no reviews for MSP/MST1 Antibody (AF352). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MSP/MST1 Antibody (AF352) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MSP/MST1 Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol MST1
Uniprot