Recombinant Human MSP (Catalog # 352‑MS) chemoattracts mouse peritoneal resident macrophages in a dose-dependent manner (orange line). The amount of cells that migrated through the filter was measured by ...read more
TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF ...read more
TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF ...read more
TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF ...read more
Mouse myeloma cell line NS0-derived recombinant human MSP/MST1 Gln19-Gly711 Accession # AAA59872
Specificity
Detects human MSP/MST1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human HGF is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MST1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for MSP/MST1 Antibody [Unconjugated]
Macrophage stimulating protein (MSP), also known as HGF-like protein, and scatter factor-2, is a member of the HGF family of growth factors (1). MSP is secreted as an inactive single chain precursor (pro-MSP) that contains a PAN/APPLE-like domain, four kringle domains, and a peptidase S1 domain which lacks enzymatic activity (2). Human MSP shares 79% aa sequence identity with mouse MSP and 44% aa sequence identity with human HGF. Pro-MSP is secreted by hepatocytes under the positive and negative control of CBP in complex with either HNF-4 or RAR, respectively (3). Circulating pro-MSP is proteolytically cleaved in response to tissue injury to yield biologically active disulfide linked heterodimers consisting of a 45‑62 kDa alpha and a 25‑35 kDa beta chain (4, 5). Pro-MSP can be activated by MT-SP1, a transmembrane protease that is expressed on macrophages and is upregulated in many cancers (6). Heterodimeric MSP as well as the isolated beta chain bind to MSP R/Ron with high-affinity, although only heterodimeric MSP can induce receptor dimerization and signaling (7, 8). MSP induces macrophage and keratinocyte proliferation and osteoclast activation (9, 10). It also inhibits LPS- or IFN-induced iNOS and IL-12 expression by macrophages and prevents apoptosis of epithelial cells separated from the ECM (11, 12).
Wang, M.-H. et al. (2002) Scand. J. Immunol. 56:545.
Han, S. et al. (1991) Biochemistry 30:9768.
Muraoka, R.S. et al. (1999) Endocrinology 140:187.
Wang, M.-H. et al. (1996) J. Clin. Invest. 97:720.
Nanney, L.B. et al. (1998) J. Invest. Dermatol. 111:573.
Bhatt, A.S. et al. (2007) Proc. Natl. Acad. Sci. 104:5771.
Wang, M.-H. et al. (1997) J. Biol. Chem. 272:16999.
Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937.
Wang, M.-H. et al. (1996) Exp. Cell Res. 226:39.
Kurihara, N. et al. (1998) Exp. Hematol. 26:1080.
Morrison, A.C. et al. (2004) J. Immunol. 172:1825.
Liu, Q.P. et al. (1999) J. Immunol. 163:6606.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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