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MOGAT2 Antibody

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Western Blot: MOGAT2 Antibody [NBP1-52078] - NBP1-52078 (0.05ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: MOGAT2 Antibody [NBP1-52078] - NBP1-52078 (0.05ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: MOGAT2 Antibody [NBP1-52078] - 3.75ug/ml staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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MOGAT2 Antibody Summary

Immunogen
Peptide with sequence C-KESAAHILNRK corresponding to internal region according to NP_079374.2.
Epitope
C-KESAAHILNRK
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MOGAT2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 0.05-0.2ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.05 ug/ml
Application Notes
Western blot: Approx 37kDa band observed in Human Colon, Duodenum and Ileum lysates (calculated MW of 38.2kDa according to NP_079374.2). Primary incubation was 1 hour
IHC: Paraffin embedded Human Small Intestine.
Publications
Read Publication using NBP1-52078.

Reactivity Notes

Expected from sequence similarity: Canine, Bovine, Porcine

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MOGAT2 Antibody

  • acyl CoA:monoacylglycerol acyltransferase 2
  • Acyl-CoA:monoacylglycerol acyltransferase 2
  • DC5
  • DGAT2L5
  • DGAT2L5MGC119183
  • Diacylglycerol acyltransferase 2-like protein 5
  • Diacylglycerol O-acyltransferase candidate 5
  • EC 2.3.1
  • EC 2.3.1.22
  • FLJ22644
  • hDC5
  • hMGAT2
  • MGAT2
  • MGAT2MGC119184
  • MGC119185
  • MOGAT2
  • monoacylglycerol O-acyltransferase 22-acylglycerol O-acyltransferase 2

Background

Dietary fat absorption from the small intestine is facilitated by acyl-CoA:monoacylglycerol transferase (MOGAT; EC 2.3.1.22) and acyl-CoA:diacylglycerol acyltransferase (DGAT; see MIM 604900) activities. MOGAT catalyzes the joining of monoacylglycerol and fatty acyl-CoAs to form diacylglycerol (Yen and Farese, 2003 [PubMed 12621063]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MOGAT2 Antibody (NBP1-52078)(1)


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Bioinformatics

Gene Symbol MOGAT2
Entrez
Uniprot