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Mind Bomb 2/MIB2 Antibody (739505)

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Western blot shows lysates of U266 human myeloma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Mind Bomb 2/MIB2 Monoclonal Antibody (Catalog # MAB72891) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Mind Bomb 2/MIB2 at approximately 103-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of U266 human myeloma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Mind Bomb 2/MIB2 Monoclonal Antibody (Catalog # MAB72891) followed by HRP-conjugated Anti-Mouse IgG ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
739505
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB72891-SP
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Mind Bomb 2/MIB2 Antibody (739505) Summary

Immunogen
E. coli-derived recombinant human Mind Bomb 2/MIB2
Ala418-Lys520
Accession # Q96AX9
Specificity
Detects human Mind Bomb 2/MIB2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MIB2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mind Bomb 2/MIB2 Antibody (739505)

  • E3 ubiquitin-protein ligase MIB2
  • EC 6.3.2
  • EC 6.3.2.-
  • EC 6.3.2.13
  • FLJ20648
  • FLJ41406
  • MIB2
  • Mind Bomb 2
  • Mind bomb homolog 2
  • Mind Bomb-2
  • mindbomb homolog 2 (Drosophila)
  • Novel zinc finger protein
  • Novelzin
  • Putative NF-kappa-B-activating protein 002N
  • SKD
  • Skeletrophin
  • Zinc finger ZZ type with ankyrin repeat domain protein 1
  • ZZ type with ankyrin repeat domain 1
  • ZZANK1
  • ZZZ5

Background

MIB-2 (Mind Bomb-2), also called skeletrophin, is a widely expressed, 1013 amino acid (aa) cytoplasmic phosphoprotein that is an E3 ubiquitin ligase for Notch ligands such as Jagged-2. It contains two MIB/HERC2 domains surrounding a ZZ-type zinc finger (aa 59-285), an ankyrin repeat domain (aa 522-856), and two RING-type zinc fingers that have E3 ubiquitin ligase activity (aa 890-1002). If short gaps in the protein sequence are ignored, human MIB-2 (aa 418-520) shares 95% and 92% aa identity with mouse and rat MIB-2, respectively. Eight alternately spliced isoforms of 672-1009 aa lack various sequences, but all contain aa 418-520. MIB-2 has been detected as a 70 kDa form in skeletal muscle, binds actin, and is downregulated in malignant melanoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MIB2
Uniprot