![Western Blot: Methionyl tRNA synthetase Antibody [NBP2-22312] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22312 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Methionyl-tRNA-synthetase-Antibody-Western-Blot-NBP2-22312-img0002.jpg "Western Blot: Methionyl tRNA synthetase Antibody [NBP2-22312] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22312 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen this antibody was made to, maps to a region between residue 175 to 225 of human Methionyl-tRNA Synthetase using the numbering given in entry NP_004981.2 (GeneID 4141). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MARS1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
![Western Blot NTAL Antibody [Unconjugated]](https://images.novusbio.com/images2/NTAL_AF4066_Western_Blot_5396.jpg)
![Intracellular Staining by Flow Cytometry NTAL Antibody [Unconjugated]](https://images.novusbio.com/images2/NTAL_AF4066_Flow_Cytometry_8223.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MARS1 |
| Uniprot |
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![Immunohistochemistry-Paraffin: Methionyl tRNA synthetase Antibody [NBP2-22312] - FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MARS (NBP2-22312) left image and (NBP2-22313) right image, used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/Methionyl-tRNA-synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-22312-img0007.jpg "Immunohistochemistry-Paraffin: Methionyl tRNA synthetase Antibody [NBP2-22312] - FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MARS (NBP2-22312) left image and (NBP2-22313) right image, used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).")
![Immunoprecipitation: Methionyl tRNA synthetase Antibody [NBP2-22312] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22312 used for IP at 6 ug/mg lysate. MARS was also immunoprecipitated by rabbit anti-MARS antibody NBP2-22313. For blotting immunoprecipitated MARS, NBP2-22312 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Methionyl-tRNA-synthetase-Antibody-Immunoprecipitation-NBP2-22312-img0003.jpg "Immunoprecipitation: Methionyl tRNA synthetase Antibody [NBP2-22312] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22312 used for IP at 6 ug/mg lysate. MARS was also immunoprecipitated by rabbit anti-MARS antibody NBP2-22313. For blotting immunoprecipitated MARS, NBP2-22312 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Immunohistochemistry CD55/DAF Antibody [Unconjugated]](https://images.novusbio.com/images2/CD55_AF2009_Immunohistochemistry_6728.jpg)
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![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot Cystatin B/Stefin B Antibody [Unconjugated]](https://images.novusbio.com/images2/Cystatin_B_AF1408_Western_Blot_16738.jpg)
![Knockout Validated Cystatin B/Stefin B Antibody [Unconjugated]](https://images.novusbio.com/images2/Cystatin_B_AF1408_Knockout_Validated_22991.jpg)
![Simple Western Cystatin B/Stefin B Antibody [Unconjugated]](https://images.novusbio.com/images2/Cystatin_B_AF1408_Simple_Western_18688.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")