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Human Methionine Aminopeptidase 2/METAP2 ELISA Kit (Colorimetric)

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ELISA: Human Methionine Aminopeptidase 2/METAP2 ELISA Kit (Colorimetric) [NBP3-39860] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Suitable Sample Type
Tissue homogenates and other biological fluids
Standard Curve Range
0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity
0.058 ng/mL (example only; lot dependent)

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Human Methionine Aminopeptidase 2/METAP2 ELISA Kit (Colorimetric) Summary

Description
Assay Length: 4.5 hours
Standard Curve Range
0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity
0.058 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
METAP2

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Reagent A
  2. Detection Reagent B
  3. Diluent Buffer
  4. Instruction manual
  5. Plate sealer for 96 wells
  6. Pre-coated 96T strip plate
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human Methionine Aminopeptidase 2/METAP2 ELISA Kit (Colorimetric)

  • Amp2
  • eIF-2-associated p67 homolog
  • Initiation factor 2-associated 67 kDa glycoprotein
  • MAP 2
  • MAP2
  • MetAP 2
  • METAP2
  • Methionine Aminopeptidase 2
  • methionyl aminopeptidase 2
  • MNPEP
  • p67
  • p67EC 3.4.11.18
  • p67eIF2
  • Peptidase M 2

Background

METAP2 is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol METAP2