Recombinant Human Methionine Aminopeptidase 1/METAP1 His Protein Summary
Description |
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-386 of Human Methionine Aminopeptidase 1/METAP1 Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAVETR VCETDGCSSE AKLQCPTCIK LGIQGSYFCS QECFKGSWAT HKLLHKKAKD EKAKREVSSW TVEGDINTDP WAGYRYTGKL RPHYPLMPTR PVPSYIQRPD YADHPLGMSE SEQALKGTSQ IKLLSSEDIE GMRLVCRLAR EVLDVAAGMI KPGVTTEEID HAVHLACIAR NCYPSPLNYY NFPKSCCTSV NEVICHGIPD RRPLQEGDIV NVDITLYRNG YHGDLNETFF VGEVDDGARK LVQTTYECLM QAIDAVKPGV RYRELGNIIQ KHAQANGFSV VRSYCGHGIH KLFHTAPNVP HYAKNKAVGV MKSGHVFTIE PMICEGGWQD ETWPDGWTAV TRDGKRSAQF EHTLLVTDTG CEILTRRLDS ARPHFMSQF |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
METAP1 |
Purity |
>90%, by SDS-PAGE |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
45.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
20 mM Tris-HCl buffer (pH 8.0), 3mM DTT, 40% glycerol, 200 mM NaCl, 0.1 mM PMSF |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
>90%, by SDS-PAGE |
Alternate Names for Recombinant Human Methionine Aminopeptidase 1/METAP1 His Protein
Background
METAP1 (Methionine aminopeptidase 1) belongs to the peptidase M24A family. This protein removes the amino-terminal methionine from nascent proteins. It required for normal progression through the cell cycle. The active site of MetAP-1 contains two adjacent divalent metal ions connected by a water molecule or hydroxide ion. Recombinant human METAP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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