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MEK1 Antibody - Azide and BSA Free

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Genetic Strategies: Western Blot: MEK1 Antibody [NBP3-02951] - Analysis of extracts from normal (control) and MAP2K1 knockout (KO) HeLa cells, using MEK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat ...read more
Immunohistochemistry-Paraffin: MEK1 Antibody [NBP3-02951] - Paraffin-embedded mouse kidney using MEK1 antibody at dilution of 1:100 (40x lens).
Western Blot: MEK1 Antibody [NBP3-02951] - Analysis of extracts of various cell lines, using MEK1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: ...read more
Immunohistochemistry: MEK1 Antibody [NBP3-02951] - Paraffin-embedded Rat brain using MEK1 antibody at dilution of 1:100 (40x lens).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Validated by:
     

Genetic Strategies

   

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Catalog# & Formulation Size Price
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MEK1 Antibody - Azide and BSA Free Summary

Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MEK1 (NP_002746.1). MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIH
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP2K1
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot 1:1000-1:3000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.01% Thimerosal
Purity
Affinity purified

Alternate Names for MEK1 Antibody - Azide and BSA Free

  • dual specificity mitogen-activated protein kinase kinase 1
  • ERK activator kinase 1
  • MAP kinase kinase 1
  • MAP2K1
  • MAPK/ERK kinase 1
  • MAPKK1
  • MEK1
  • MEK1MAPKK 1
  • mitogen-activated protein kinase kinase 1
  • MKK1
  • MKK1MEK 1
  • PRKMK1EC 2.7.12.2
  • protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Background

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992) Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Bioinformatics

Gene Symbol MAP2K1