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MEK2 Antibody (OTI1A2)

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Western Blot: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western Blot: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; ...read more
Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK2.
Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescence: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MEK2.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1A2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72647AF350
Alexa Fluor 405
NBP2-72647AF405
Alexa Fluor 488
NBP2-72647AF488
Alexa Fluor 532
NBP2-72647AF532
Alexa Fluor 594
NBP2-72647AF594
Alexa Fluor 647
NBP2-72647AF647
Alexa Fluor 700
NBP2-72647AF700
Alexa Fluor 750
NBP2-72647AF750
Allophycocyanin
NBP2-72647APC
Biotin
NBP2-72647B
DyLight 350
NBP2-72647UV
DyLight 405
NBP2-72647V
DyLight 488
NBP2-72647G
DyLight 550
NBP2-72647R
DyLight 594
NBP2-72647DL594
DyLight 650
NBP2-72647C
DyLight 680
NBP2-72647FR
DyLight 755
NBP2-72647IR
FITC
NBP2-72647F
HRP
NBP2-72647H
Janelia Fluor 549
NBP2-72647JF549
Janelia Fluor 646
NBP2-72647JF646
PE
NBP2-72647PE
PerCP
NBP2-72647PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-72647
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MEK2 Antibody (OTI1A2) Summary

Immunogen
Full length human recombinant protein of human MAP2K2(NP_109587) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MAP2K2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:200-4000
Theoretical MW
44.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MEK2 Antibody (OTI1A2)

  • ERK activator kinase 2
  • MAP2K2
  • MAPKK 2
  • MEK 2
  • MEK2
  • MEK2EC 2.7.12.2
  • mitogen-activated protein kinase kinase 2
  • MKK2
  • MKK2FLJ26075
  • p45
  • PRKMK2MAPKK2

Background

MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MAP2K2