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ME1 Antibody (15611)

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Western Blot: ME1 Antibody (15611) [NBP2-43669] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody [15611].
Genetic Strategies: Western Blot: ME1 Antibody (15611) [NBP2-43669] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ...read more
Immunohistochemistry-Frozen: ME1 Antibody (15611) [NBP2-43669] - Vimentin antibody (NBP1-31327) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Red: ME1 antibody 15611] ...read more
Western Blot: ME1 Antibody (15611) [NBP2-43669] - Analysis of various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody diluted at a dilution of 1:1000.
Immunohistochemistry-Paraffin: ME1 Antibody (15611) [NBP2-43669] - Analysis of mouse cervix. ME1 antibody [15611] diluted at 1:500.
Immunohistochemistry-Paraffin: ME1 Antibody (15611) [NBP2-43669] - Analysis of mouse liver. ME1 antibody [15611] diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ShSpecies Glossary
Applications WB, IHC
Clone
15611
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

    

Order Details

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ME1 Antibody (15611) Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ME1. The exact sequence is proprietary.
Predicted Species
Rat (91%), Sheep (92%), Bovine (91%). Backed by our 100% Guarantee.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ME1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for ME1 Antibody (15611)

  • EC 1.1.1
  • EC 1.1.1.40
  • HUMNDME
  • malate dehydrogenase
  • Malic enzyme 1
  • malic enzyme 1, NADP(+)-dependent, cytosolic
  • malic enzyme 1, soluble
  • Malic enzyme, cytoplasmic
  • MES
  • NADP-dependent malic enzyme
  • NADP-ME
  • pyruvic-malic carboxylase

Background

ME1 encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-43669
ME1 Antibody (15611)
Species: Hu, Mu, Rt, Bv, Sh
Applications: WB, IHC

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Bioinformatics

Gene Symbol ME1