![Immunocytochemistry/Immunofluorescence: MDR1/ABCB1 Antibody [NBP2-17251] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: P Glycoprotein protein stained by P Glycoprotein antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17251-img0003.jpg "Immunocytochemistry/Immunofluorescence: MDR1/ABCB1 Antibody [NBP2-17251] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: P Glycoprotein protein stained by P Glycoprotein antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
| Reactivity | Hu, Fe, Rb, RM, ShSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MDR1/ABCB1. The exact sequence is proprietary. |
| Predicted Species | Sheep (93%), Feline (92%), Rhesus Macaque (93%), Rabbit (93%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ABCB1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 141 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
| Publication using NBP2-17251 | Applications | Species |
|---|---|---|
| Huang R, Zhu L, Zhang Y XIST lost induces ovarian cancer stem cells to acquire taxol resistance via a KMT2C-dependent way Cancer cell international 2020-09-04 [PMID: 32943985] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for MDR1/ABCB1 Antibody (NBP2-17251)Find related products by research area.
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![Western Blot: MDR1/ABCB1 Antibody [NBP2-17251] - Unboiled HepG2 whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MDR1/ABCB1 antibody (NBP2-17251) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)](http://images.novusbio.com/fullsize/nbp2-17251_rabbit-polyclonal-mdr1-abcb1-antibody-910202419593571.jpg "Western Blot: MDR1/ABCB1 Antibody [NBP2-17251] - Unboiled HepG2 whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MDR1/ABCB1 antibody (NBP2-17251) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)")
![PXR/NR1I2 Antibody (H4417) [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "PXR/NR1I2 Antibody (H4417) [Unconjugated]")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")