![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Analysis in human adrenal gland and skeletal muscle tissues. Corresponding MDR1/ABCB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0009.jpg "Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Analysis in human adrenal gland and skeletal muscle tissues. Corresponding MDR1/ABCB1 RNA-seq data are presented for the same tissues.")
| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: IAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTAGNEVELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKLSTKEALDE |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ABCB1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publications using NBP1-90291 | Applications | Species |
|---|---|---|
| Lehmann V The Modern Day Heracles: Patient-derived Liver Organoids to Model Rare Pediatric Liver Diseases Thesis 2023-01-01 | ||
| KrUger M, Samsom RA, Oosterhoff LA et al. High level of polarized engraftment of porcine intrahepatic cholangiocyte organoids in decellularized liver scaffolds Journal of cellular and molecular medicine 2022-08-26 [PMID: 36017767] (IHC-P, Porcine) Details: IHC-P 1:200 |
IHC-P | Porcine |
| Trumpi K, Emmink BL, Prins AM et al. ABC-Transporter Expression Does Not Correlate with Response to Irinotecan in Patients with Metastatic Colorectal Cancer. J Cancer 2015-01-01 [PMID: 26516354] (IF/IHC, Human) | IF/IHC | Human |
| Bernstein HG, Holzl G, Dobrowolny H et al. Vascular and extravascular distribution of the ATP-binding cassette transporters ABCB1 and ABCC1 in aged human brain and pituitary. Mech Ageing Dev 2014-09-10 [PMID: 25218792] (IF/IHC, Human) | IF/IHC | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for MDR1/ABCB1 Antibody (NBP1-90291)Find related products by research area.
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| Gene Symbol | ABCB1 |
![Immunocytochemistry/Immunofluorescence: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human cell line A-431 shows localization to nucleoplasm & focal adhesion sites. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90291-img0004.jpg "Immunocytochemistry/Immunofluorescence: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human cell line A-431 shows localization to nucleoplasm & focal adhesion sites. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human small intestine shows moderate positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0014.jpg "Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human small intestine shows moderate positivity in apical membrane in glandular cells.")
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human adrenal gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0010.jpg "Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human adrenal gland shows strong membranous positivity in glandular cells.")
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human kidney shows moderate positivity in apical membrane in cells in distal tubules.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0011.jpg "Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human kidney shows moderate positivity in apical membrane in cells in distal tubules.")
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human liver shows weak membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0012.jpg "Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human liver shows weak membranous positivity in hepatocytes.")
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0013.jpg "Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human skeletal muscle shows no positivity in myocytes as expected.")
![PXR/NR1I2 Antibody (H4417) [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "PXR/NR1I2 Antibody (H4417) [Unconjugated]")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")