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MDC1 Antibody

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Western Blot: MDC1 Antibody [NB100-56657] - Analysis of MDC1 in 30 ugs of Jurkat cell lysate using this antibody at 1:1000 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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MDC1 Antibody Summary

Immunogen
This antibody was developed against a synthetic peptide corresponding to amino acids 871-888 (RQESDKNGESASPERDRE) of human MDC1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MDC1
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:1000
Application Notes
In Jurkat, an approx. 220 kDa band is observed.
Control
Jurkat Whole Cell Lysate
Publications
Read Publication using NB100-56657.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for MDC1 Antibody

  • DKFZp781A0122
  • Em:AB023051.5
  • KIAA0170MGC166888
  • MDC1
  • mediator of DNA damage checkpoint 1
  • mediator of DNA damage checkpoint protein 1
  • mediator of DNA-damage checkpoint 1
  • NFBD1
  • NFBD1homologue to Drosophila photoreceptor protein calphotin
  • Nuclear factor with BRCT domains 1

Background

MDC1, nuclear factor with BRCT domains protein 1, participates in the early response to DNA damage. It is thought to be a member of the BRCT (BRCA1 C-terminus) superfamily of nuclear proteins. It contains an N-terminus forkhead-associated (FHA) motif, two C-terminus BRCT motifs and 13 internal repetitions of a 41 amino acid sequence. NFBD1 is an alternate name for MDC1 (mediator of DNA damage checkpoint protein).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MDC1 Antibody (NB100-56657)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional MDC1 Products

Array NB100-56657

Research Areas for MDC1 Antibody (NB100-56657)

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Blogs on MDC1.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

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Bioinformatics

Gene Symbol MDC1
Entrez
Uniprot