![Western Blot: Mcl-1 Antibody (CL1128) [NBP2-52968] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: MCL1 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)](http://images.novusbio.com/fullsize/Mcl-1-Antibody-CL1128-Western-Blot-NBP2-52968-img0005.jpg "Western Blot: Mcl-1 Antibody (CL1128) [NBP2-52968] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: MCL1 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | CL1128 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: DAIMSPEEELDGYEPEPLGKRPAVLPLLELVGESGNNTSTDGSLPSTPPPAEEEEDELYRQSLEIISRYLREQATGAKDTKPMGRSGATSRKALETLRRVGDG |
| Epitope | PEEELDGYEP |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MCL1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. |
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| Control Peptide |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Protein A purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Kristen Gumz |
ELISA | Human | 04/28/2017 |
Summary
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MCL1 |
![Immunocytochemistry/Immunofluorescence: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of U-2 OS cells using the Anti-MCL1 monoclonal antibody, showing cell cycle dependent staining in mitochondria in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Mcl-1-Antibody-CL1128-Immunocytochemistry-Immunofluorescence-NBP2-52968-img0007.jpg "Immunocytochemistry/Immunofluorescence: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of U-2 OS cells using the Anti-MCL1 monoclonal antibody, showing cell cycle dependent staining in mitochondria in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).")
![Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of lymph node in human colon shows strong positivity in the reaction centrum.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-CL1128-Immunohistochemistry-Paraffin-NBP2-52968-img0006.jpg "Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of lymph node in human colon shows strong positivity in the reaction centrum.")
![Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of human breast cancer shows strong positivity in tumor cells.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-CL1128-Immunohistochemistry-Paraffin-NBP2-52968-img0001.jpg "Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of human breast cancer shows strong positivity in tumor cells.")
![Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of human cerebellum shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/Mcl-1-Antibody-CL1128-Immunohistochemistry-Paraffin-NBP2-52968-img0002.jpg "Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of human cerebellum shows absence of immunoreactivity (negative control).")
![Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of human tonsil shows strong immunoreactivity in the reaction centrum.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-CL1128-Immunohistochemistry-Paraffin-NBP2-52968-img0003.jpg "Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of human tonsil shows strong immunoreactivity in the reaction centrum.")
![Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of stomach cancer shows cytoplasmic immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-CL1128-Immunohistochemistry-Paraffin-NBP2-52968-img0004.jpg "Immunohistochemistry-Paraffin: Mcl-1 Antibody (CL1128) [NBP2-52968] - Staining of stomach cancer shows cytoplasmic immunoreactivity in tumor cells.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")