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MAT2A Antibody

Images

 
Western Blot: MAT2A Antibody [NBP1-92100] - Analysis in human cell line CAPAN-2.
Immunocytochemistry/ Immunofluorescence: MAT2A Antibody [NBP1-92100] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100] - Staining of human Liver shows very weak nuclear positivity in hepatocytes.
Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100] - Staining of human Duodenum shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100] - Staining of human Kidney shows strong nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100] - Staining of human Prostate shows strong nuclear positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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MAT2A Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MNGQLNGFHEAFIEEGTFL
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAT2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval method is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MAT2A Protein (NBP1-92100PEP)
Publications
Read Publications using
NBP1-92100 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30926424).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MAT2A Antibody

  • AdoMet synthase 2
  • AMS2
  • EC 2.5.1.6
  • MAT 2
  • MAT2A
  • MATA2
  • MATA2SAMS2adoMet synthetase 2
  • MATII
  • MAT-II
  • Methionine adenosyltransferase 2
  • Methionine adenosyltransferase II
  • methionine adenosyltransferase II, alpha
  • S-adenosylmethionine synthase isoform type-2
  • S-adenosylmethionine synthetase isoform type-2
  • SAMS2

Background

MAT1A and MAT2A are catalytic subunits of methionine adenosyltransferase (MAT), which catalyzes the formation of S-adenosyltransferase for methionine catabolism. Inactivation of MAT1A has been linked to liver disease. In addition, in certain liver cancers, expression of MAT1A is replaced by expression of MAT1B. Overexpression of MAT2A has also been observed in colon cancer, leading to the proposal that MAT2A facilitates cancer cell growth.

MAT1A and MAT2A antibodies are useful tools for cancer research and liver function research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MAT2A Antibody (NBP1-92100)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IF/IHC, IHC-P, WB.


Filter By Application
IF/IHC
(2)
IHC-P
(1)
WB
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAT2A Antibody (NBP1-92100) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MAT2A Products

Research Areas for MAT2A Antibody (NBP1-92100)

Find related products by research area.

Blogs on MAT2A.

MAT2A - A Protien Coding Gene
The enzyme S-adenosylmethionine synthetase, or MAT, catalyzes the formation of S- adenosylmethionine (AdoMet or SAMe) from methionine and ATP. AdoMet is the principal biological source of synthesized methyl, and is found in all cells but most prominen...  Read full blog post.

MAT2a, MAT2b, HIF-1 alpha: Roles in Liver Cancer and DNA methylation
Methionine Adenosyltransferase II alpha, also known as MAT2a, is a catalytic subunit of methionine adenosyltransferase (MAT) and essential enzyme for the catalysis of the principle biological methyl donor, S-adenosylmethionine (SAM) from methionine an...  Read full blog post.

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Bioinformatics

Gene Symbol MAT2A
Entrez
Uniprot