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![Immunocytochemistry/Immunofluorescence: MAT2A Antibody [NB110-94158] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-MAT2A Antibody (NB110-94158) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/MAT2A-Antibody-Immunocytochemistry-Immunofluorescence-NB110-94158-img0010.jpg "Immunocytochemistry/Immunofluorescence: MAT2A Antibody [NB110-94158] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-MAT2A Antibody (NB110-94158) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: MAT2A Antibody [NB110-94158] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-MAT2A Antibody (NB110-94158) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/MAT2A-Antibody-Immunocytochemistry-Immunofluorescence-NB110-94158-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: MAT2A Antibody [NB110-94158] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-MAT2A Antibody (NB110-94158) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/MAT2A-Antibody-Immunocytochemistry-Immunofluorescence-NB110-94158-img0012.jpg)
![Western Blot: MAT2A Antibody [NB110-94158] - Loss of PKM2 impairs methylation capacity, reduces DNA methylation and leads to the expression of endogenous retroviral elements. Western blot analysis of MAT2A, P53, P21 and PKM2 in control, P53KD, PKM2KD and PKM2KD/P53KD ECs. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-06406-8), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MAT2A-Antibody-Western-Blot-NB110-94158-img0008.jpg)
![Immunohistochemistry-Paraffin: MAT2A Antibody [NB110-94158] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of MAT2A antibody (NB110-94158). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/MAT2A-Antibody-Immunohistochemistry-Paraffin-NB110-94158-img0009.jpg)
![Western Blot: MAT2A Antibody [NB110-94158] - Total protein from HeLa and HepG2 cells and Human and Mouse Kidney was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-MAT2A in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/MAT2A-Antibody-Western-Blot-NB110-94158-img0005.jpg)
![Western Blot: MAT2A Antibody [NB110-94158] - Detection of MAT2A in HepG2 whole cell lysates.](http://images.novusbio.com/fullsize/MAT2A-Antibody-Western-Blot-NB110-94158-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MAT2A Antibody [NB110-94158] - MAT2A antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/MAT2A-Antibody-Immunocytochemistry-Immunofluorescence-NB110-94158-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: MAT2A Antibody [NB110-94158] - Mouse embryonic stem cells (mESCs) were fixed with paraformaldehyde, permeabilized with Triton, blocked with 4% BSA and 1% normal goat serum, and incubated with 1:200 dilution of MAT2A antibody. Then, samples were stained with anti-rabbit Alexa Fluor 594 (1:1000) and NucBlue. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/MAT2A-Antibody-Immunocytochemistry-Immunofluorescence-NB110-94158-img0006.jpg)
![Simple Western: MAT2A Antibody [NB110-94158] - Human HepG2 cell lysate (0.2 ug/uL total protein). Antibody at 1:250. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/MAT2A-Antibody-Simple-Western-NB110-94158-img0007.jpg)
![Western Blot: MAT1/2A Antibody [NB110-94162] - Detection of MAT2A in HepG2 whole cell lysates using NB110-94162.](http://images.novusbio.com/fullsize/MAT1-2A-Antibody-Western-Blot-NB110-94162-img0003.jpg "Western Blot: MAT1/2A Antibody [NB110-94162] - Detection of MAT2A in HepG2 whole cell lysates using NB110-94162.")
![Simple Western: MAT1/2A Antibody [NB110-94162] - Simple Western lane view shows a specific band for MAT1/2 A in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/MAT1-2A-Antibody-Simple-Western-NB110-94162-img0004.jpg)
![Western Blot: MAT2A Antibody [NBP1-92100] - Analysis in human cell line CAPAN-2.](http://images.novusbio.com/fullsize/MAT2A-Antibody-Western-Blot-NBP1-92100-img0009.jpg "Western Blot: MAT2A Antibody [NBP1-92100] - Analysis in human cell line CAPAN-2.")
![Immunocytochemistry/Immunofluorescence: MAT2A Antibody [NBP1-92100] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MAT2A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92100-img0007.jpg)
![Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100] - Staining of human Liver shows very weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/MAT2A-Antibody-Immunohistochemistry-Paraffin-NBP1-92100-img0012.jpg)
![Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100] - Staining of human Duodenum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/MAT2A-Antibody-Immunohistochemistry-Paraffin-NBP1-92100-img0010.jpg)
![Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100] - Staining of human Kidney shows strong nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/MAT2A-Antibody-Immunohistochemistry-Paraffin-NBP1-92100-img0011.jpg)
![Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100] - Staining of human Prostate shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/MAT2A-Antibody-Immunohistochemistry-Paraffin-NBP1-92100-img0013.jpg)
![Western Blot: MAT2A Antibody (3A2) [NBP1-28605] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAT2A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HeLa cell lysateLane 2. : Ramos cell lysateLane 3. : 293T cell lysateLane 4. : Jurkat cell lysateLane 5. : NIH-3T3 cell lysate](http://images.novusbio.com/fullsize/MAT2A-Antibody-3A2-Western-Blot-NBP1-28605-img0006.jpg "Western Blot: MAT2A Antibody (3A2) [NBP1-28605] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAT2A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HeLa cell lysateLane 2. : Ramos cell lysateLane 3. : 293T cell lysateLane 4. : Jurkat cell lysateLane 5. : NIH-3T3 cell lysate")
![Immunocytochemistry/Immunofluorescence: MAT2A Antibody (3A2) [NBP1-28605] - Analysis of MAT2A in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MAT2A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/MAT2A-Antibody-3A2-Immunocytochemistry-Immunofluorescence-NBP1-28605-img0007.jpg)
![Immunohistochemistry-Paraffin: MAT2A Antibody (3A2) [NBP1-28605] - Human MAT2A (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).](http://images.novusbio.com/fullsize/MAT2A-Antibody-3A2-Immunohistochemistry-Paraffin-NBP1-28605-img0003.jpg)
![Western Blot: MAT2A Antibody (3A2) [NBP1-28605] - Cell lysates of HepG2 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MAT2A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/MAT2A-Antibody-3A2-Western-Blot-NBP1-28605-img0004.jpg)
![Western Blot: MAT1/2A Antibody (HL1679) - Azide and BSA Free [NBP3-25557] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MAT1A + MAT2A antibody [HL1679] (NBP3-25557) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25557_rabbit-mat1-2a-mab-hl1679-azide-and-bsa-free-212024940892.jpg "Western Blot: MAT1/2A Antibody (HL1679) - Azide and BSA Free [NBP3-25557] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MAT1A + MAT2A antibody [HL1679] (NBP3-25557) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: MAT1/2A Antibody (HL1679) - Azide and BSA Free [NBP3-25557] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with MAT1A + MAT2A antibody [HL1679] (NBP3-25557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25557_rabbit-mat1-2a-mab-hl1679-azide-and-bsa-free-2120249383121.jpg)
![Western Blot: MAT1/2A Antibody (HL1679) - Azide and BSA Free [NBP3-25557] - 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MAT1A + MAT2A antibody [HL1679] (NBP3-25557) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25557_rabbit-mat1-2a-mab-hl1679-azide-and-bsa-free-2120249415951.jpg)
![Western Blot: MAT1/2A Antibody (HL1679) - Azide and BSA Free [NBP3-25557] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MAT1A + MAT2A antibody [HL1679] (NBP3-25557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25557_rabbit-mat1-2a-mab-hl1679-azide-and-bsa-free-2120249355542.jpg)
![Western Blot: MAT2A Antibody (7A0X1) [NBP3-15730] - Western blot analysis of extracts of various cell lines, using MAT2A antibody (NBP3-15730) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/MAT2A-Antibody-7A0X1-Western-Blot-NBP3-15730-img0001.jpg "Western Blot: MAT2A Antibody (7A0X1) [NBP3-15730] - Western blot analysis of extracts of various cell lines, using MAT2A antibody (NBP3-15730) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: MAT2A Antibody (7A0X1) [NBP3-15730] - Western blot analysis of extracts of HepG2 cells, using MAT2A antibody (NBP3-15730) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/MAT2A-Antibody-7A0X1-Western-Blot-NBP3-15730-img0002.jpg)
![Western Blot: MAT2A Antibody (SR1837) [NBP3-22383] - Western blot analysis using NBP3-22383 in (1) Ramos cell lysate;(2) Mouse spleen lysate.](http://images.novusbio.com/fullsize/nbp3-22383_rabbit-mat2a-mab-sr1837-57202316582170.jpg "Western Blot: MAT2A Antibody (SR1837) [NBP3-22383] - Western blot analysis using NBP3-22383 in (1) Ramos cell lysate;(2) Mouse spleen lysate.")
![Western Blot: MAT2A Antibody [NBP2-32283] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-MAT2A antibody NBP2-32283 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MAT2A-Antibody-Western-Blot-NBP2-32283-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: MAT2A Antibody [NBP2-32283] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-MAT2A antibody NBP2-32283 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: MAT2A Antibody [NBP3-04713] - Analysis of extracts of various cell lines, using MAT2A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/MAT2A-Antibody-Western-Blot-NBP3-04713-img0001.jpg "Western Blot: MAT2A Antibody [NBP3-04713] - Analysis of extracts of various cell lines, using MAT2A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Western Blot: MAT2A Overexpression Lysate (Adult Normal) [NBL1-12910] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAT2A.](http://images.novusbio.com/fullsize/MAT2A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12910-img0002.jpg "Western Blot: MAT2A Overexpression Lysate (Adult Normal) [NBL1-12910] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAT2A.")